AN EQUILIBRIUM ULTRACENTRIFUGE STUDY OF SELF-ASSOCIATION OF BOVINE INSULIN
AN EQUILIBRIUM ULTRACENTRIFUGE STUDY OF SELF-ASSOCIATION OF BOVINE INSULIN
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DOI：
10.1021/bi00866a014
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发表时间：
1966-01-01
期刊：
影响因子：
2.9
通讯作者：
COATES, JH
中科院分区：
文献类型：
--
作者：
JEFFREY, PD;COATES, JH
胰岛素在水溶液中自缔合。通过沉降平衡实验测得单体的分子量为5800awu。在pH2时，离子强度为0. 1，单体，二聚体，四聚体和六聚体存在于动态平衡。在15和25[degree]下的测量产生k。缔合反应的[DELTA]Go、[DELTA] Ho和[DELTA]So值。统计力学计算表明，有限的旋转自由度的单体伴随着疏水键的假设帐户最令人满意的观察到的熵的关联。
Insulin self-associ-ates in aqueous solution. By sedimentations equilibrium experiments the monomer molecular weight is found to be 5800 awu. At pH 2, ionic strength o. 1, monomers, dimers, tetramer, and hexamers are found to exist in dynamic equilibrium. Measurements at 15 and 25[degree] yield k.[DELTA]Go, [DELTA] Ho, and [DELTA]So values for the association reactions. Statistical mechanical calculations suggest that the hypothesis of limited rotational freedom of the monomers accompanied by hydrophobic bonding accounts most satisfactorily for the observed entropies of association.