Selective reentry of recycling cell surface glycoproteins to the biosynthetic pathway in human hepatocarcinoma HepG2 cells
Selective reentry of recycling cell surface glycoproteins to the biosynthetic pathway in human hepatocarcinoma HepG2 cells
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人肝癌 HepG2 细胞中回收细胞表面糖蛋白选择性重新进入生物合成途径
DOI：
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发表时间：
1995
影响因子：
7.8
通讯作者：
Rudolf Tauber
中科院分区：
文献类型：
--
作者：
Barbara Volz;G. Orberger;S. Porwoll;Hans;Rudolf Tauber
已经在HepG 2细胞中检查了细胞表面糖蛋白返回到分泌途径的隔室，比较返回到反式高尔基体网络（TGN）、反式/中间和顺式高尔基体。运输到这些网站进行了研究，例如转铁蛋白受体（TfR）和丝氨酸肽酶二肽基肽酶IV（DPPIV）标记后，这些蛋白质与N-羟基磺基琥珀酰亚胺酯的生物素在细胞表面上。该实验设计允许区分从细胞表面返回到这些生物合成隔室的糖蛋白和在生物合成过程中通过这些隔室到达表面的新合成的糖蛋白。测量TGN的再进入，因为表面糖蛋白用神经氨酸酶去唾液酸化，并在再循环期间监测再唾液酸化。通过岩藻糖基转移酶测量[3 H]岩藻糖残基向再循环表面蛋白的转移，追踪返回到反式高尔基体。为了研究返回到顺式高尔基体，在甘露糖苷酶I抑制剂脱氧mannojirimycin（dMM）的存在下代谢标记表面蛋白。因此，表面蛋白保留寡甘露糖苷型的N-聚糖。返回到内侧/顺式高尔基体中的甘露糖苷酶I位点，监测dMM洗脱后这些聚糖向复合型聚糖的转化。我们的数据表明，DPPIV不仅从细胞表面返回到TGN，而且还返回到trans-Golgi，从而将内吞途径与分泌途径连接起来。与此相反，没有再进入甘露糖苷酶I的网站可以检测到，表明早期分泌途径是不是或只是在微不足道的速度可达循环DPPIV。与DPPIV相反，TfR非常有效地从内体分选到细胞表面，并且不会以可检测的量返回到TGN或其他生物合成区室，这表明单个表面蛋白在再循环期间受到不同的分选机制或分选效率的影响。
Return of cell surface glycoproteins to compartments of the secretory pathway has been examined in HepG2 cells comparing return to the trans- Golgi network (TGN), the trans/medial- and cis-Golgi. Transport to these sites was studied by example of the transferrin receptor (TfR) and the serine peptidase dipeptidylpeptidase IV (DPPIV) after labeling these proteins with the N-hydroxysulfosuccinimide ester of biotin on the cell surface. This experimental design allowed to distinguish between glycoproteins that return to these biosynthetic compartments from the cell surface and newly synthesized glycoproteins that pass these compartments during biosynthesis en route to the surface. Reentry to the TGN was measured in that surface glycoproteins were desialylated with neuraminidase and were monitored for resialylation during recycling. Return to the trans-Golgi was traced measuring the transfer of [3H]fucose residues to recycling surface proteins by fucosyltransferases. To study return to the cis-Golgi, surface proteins were metabolically labeled in the presence of the mannosidase I inhibitor deoxymannojirimycin (dMM). As a result surface proteins retained N-glycans of the oligomannosidic type. Return to the site of mannosidase I in the medial/cis-Golgi was measured monitoring conversion of these glycans to those of the complex type after washout of dMM. Our data demonstrate that DPPIV does return from the cell surface not only to the TGN, but also to the trans-Golgi thus linking the endocytic to the secretory pathway. In contrast, no reentry to sites of mannosidase I could be detected indicating that the early secretory pathway is not or is only at insignificant rates accessible to recycling DPPIV. In contrast to DPPIV, TfR was very efficiently sorted from endosomes to the cell surface and did not return to the TGN or to other biosynthetic compartments in detectable amounts, indicating that individual surface proteins are subject to different sorting mechanisms or sorting efficiencies during recycling.
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DOI：
10.1016/s0021-9258(17)44551-0
发表时间：
1983-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Ciechanover;A. Schwartz;A. Dautry‐Varsat;H. Lodish
通讯作者：
A. Ciechanover;A. Schwartz;A. Dautry‐Varsat;H. Lodish
重组为蛋白脂质体并部分纯化高尔基体膜 UDP-半乳糖、UDP-木糖和 UDP-葡萄糖醛酸转运活性。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Milla,ME;Clairmont,CA;Hirschberg,CB
通讯作者：
Hirschberg,CB
影响因子：
2.9
作者：
R. B. Trimble;Frank Maley
通讯作者：
R. B. Trimble;Frank Maley
YTRF 是转铁蛋白受体的保守内化信号，第 31-34 位的第二个 YTRF 信号可增强内吞作用。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Collawn,JF;Lai,A;Domingo,D;Fitch,M;Hatton,S;Trowbridge,IS
通讯作者：
Trowbridge,IS
在培养的人体细胞中将表面甘露糖 6-磷酸受体转运至高尔基复合体。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jin,M;SahagianJr,GG;Snider,MD
通讯作者：
Snider,MD