Microglia enhanced the angiogenesis, migration and proliferation of co-cultured RMECs.
Microglia enhanced the angiogenesis, migration and proliferation of co-cultured RMECs.
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小胶质细胞增强了共培养 RMEC 的血管生成、迁移和增殖。
DOI：
10.1186/s12886-018-0886-z
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发表时间：
2018-09-17
影响因子：
2
通讯作者：
Wu H
中科院分区：
文献类型：
--
作者：
Ding X;Gu R;Zhang M;Ren H;Shu Q;Xu G;Wu H
越来越多的注意力被给予小胶质细胞相关的炎症在新生血管疾病，如糖尿病视网膜病变和年龄相关性黄斑疾病。有证据表明，激活的小胶质细胞有助于破坏血-视网膜屏障，但其机制尚不清楚。本研究旨在阐明小胶质细胞是否以及如何影响视网膜微血管内皮细胞（RMEC）的功能。我们通过脂多糖（LPS）刺激激活小胶质细胞。在使用Transwell系统将静止或活化的小胶质细胞与RMEC共培养后，我们评估RMEC的功能。ELISA法检测下室上清液中血管内皮生长因子-A（VEGF-A）和血小板衍生生长因子-BB（PDGF-BB）的水平。血管生成，迁移和RMEC的增殖进行了评估管形成，伤口愈合，和WST-1测定。Western blot分析紧密连接蛋白ZO-1和occludin的表达水平以及内皮标志物CD 31和CD 34的表达水平。我们成功地建立了LPS激活的小胶质细胞模型和静态或激活的小胶质细胞与RMEC的共培养体系。在共培养系统中，我们发现小胶质细胞，特别是活化的小胶质细胞刺激VEGF-A和PDGF-BB的表达，增强血管生成，迁移，增殖和渗透性，并改变共培养RMEC的表型。小胶质细胞，尤其是活化的小胶质细胞，在视网膜微血管的血管生成和维持血管功能止血中起重要作用。其机理有待进一步研究和澄清。
Attention is increasingly being given to microglia-related inflammation in neovascular diseases, such as diabetic retinopathy and age-related macular disease. Evidence shows that activated microglia contribute to disruption of the blood–retinal barrier, however, the mechanism is unclear. In this study, we aimed to clarify whether and how microglia affect the function of retinal microvascular endothelial cells (RMECs). We activated microglia by Lipopolysaccharides (LPS) stimulation. After co-culturing static or activated microglia with RMECs using the Transwell system, we evaluated the function of RMECs. Vascular endothelial growth factor-A (VEGF-A) and platelet-derived growth factor-BB (PDGF-BB) levels in the supernatant from the lower chamber were evaluated by ELISA. Angiogenesis, migration, and proliferation of RMECs were assessed by tube formation, wound healing, and WST-1 assays. The expression levels of tight junction proteins (ZO-1 and occludin) and endothelial markers (CD31 and CD34) were examined by Western blot analysis. We successfully established an LPS-activated microglia model and co-culture system of static or activated microglia with RMECs. In the co-culture system, we showed that microglia, especially activated microglia stimulated VEGF-A and PDGF-BB expression, enhanced angiogenesis, migration, proliferation, and permeability, and altered the phenotype of co-cultured RMECs. Microglia, especially activated microglia, play important roles in angiogenesis and maintenance of vascular function hemostasis in the retinal microvasculature. The mechanism needs further investigation and clarification.
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影响因子：
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