Methods to Study Drug Uptake at the Blood-Brain Barrier Following Experimental Ischemic Stroke: In Vitro and In Vivo Approaches.
Methods to Study Drug Uptake at the Blood-Brain Barrier Following Experimental Ischemic Stroke: In Vitro and In Vivo Approaches.
复制标题
研究实验性缺血性中风后血脑屏障药物摄取的方法：体外和体内方法。
DOI：
10.1007/978-1-0716-2926-0_28
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Ronaldson,PatrickT
中科院分区：
文献类型：
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作者：
Betterton,RobertD;Williams,EricaI;Nilles,KelsyL;Davis,ThomasP;Ronaldson,PatrickT
药物对血脑屏障(BBB)的通透性是开发治疗缺血性中风等神经系统疾病的治疗策略的重要概念。这些机制可以通过使用培养的脑微血管内皮细胞或遭受实验性中风的完整动物来详细评估。在这里，我们描述了使用体外和体内方法研究治疗药物血脑屏障转运的最先进方法。这些方法允许精确确定目前市场上销售的治疗药物或正在开发中的中风药物的新化学实体的转运蛋白动力学特性。
Drug permeability across the blood-brain barrier (BBB) is an important concept in the development of therapeutic strategies to treat neurological diseases such as ischemic stroke. These mechanisms can be evaluated in detail using cultured brain microvascular endothelial cells or intact animals subjected to experimental stroke. Here, we describe state-of-the-art approaches to study BBB transport of therapeutics using our in vitro and in vivo approaches. These methodologies allow for precise determination of transporter kinetic properties for currently marketed therapeutics or for new chemical entities that are under development as stroke drugs.