Evidence supporting antioxidant action of adipose-derived stem cells: Protection of human dermal fibroblasts from oxidative stress
Evidence supporting antioxidant action of adipose-derived stem cells: Protection of human dermal fibroblasts from oxidative stress
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DOI：
10.1016/j.jdermsci.2007.08.004
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发表时间：
2008-02-01
影响因子：
4.6
通讯作者：
Sung, Jong-Hyuk
中科院分区：
文献类型：
--
作者：
Kim, Won-Serk;Park, Byung-Soon;Sung, Jong-Hyuk
背景：皮下脂肪组织基质血管部分的间充质干细胞，脂肪源性干细胞（ADSCs），产生可溶性因子，并在皮肤生物学中表现出多种药理作用。目的：研究ADSCs通过叔丁基的抗氧化作用对人真皮成纤维细胞的保护作用。过氧化氢（tbOOH）诱导氧化损伤模型。方法与结果：采集ADSCs条件培养基（ADSC-CM），检测其抗氧化作用。ADSC-CM具有100 μ M抗坏血酸的抗氧化作用，并通过蛋白质组学分析检测到多种抗氧化蛋白。形态学变化和细胞存活实验表明，与ADSC-CM孵育有助于HDFs抵抗tbOOH诱导的自由基。此外，ADSC-CM处理的HDFs中超氧化物歧化酶和谷胱甘肽过氧化物酶活性增强，证实了adsc通过抗氧化作用保护HDFs的研究假设。在细胞周期分析中，ADSC-CM处理逆转了tbOOH诱导的凋亡细胞死亡，并且与未处理的细胞相比，导致亚g1细胞减少。caspase-3活性测定也证实了ADSC-CM的抗凋亡作用。结论：ADSCs具有较强的抗氧化活性，可通过减少凋亡细胞来保护HDFs免受氧化损伤。因此，ADSCS和ADSC-CM是控制和预防各种皮肤状况下自由基对皮肤损伤的良好候选者。(c) 2007年日本皮肤病调查学会。爱思唯尔爱尔兰有限公司出版。版权所有。
Background: Mesenchymal stem cells within the stromal-vascular fraction of subcutaneous adipose tissue, adipose-derived stem cells (ADSCs), produced soluble factors and they exhibit diverse pharmacological effects in skin biology. Objective: The present study examines the protective effect of ADSCs for human dermal fibroblasts (HDFs) through anti-oxidation in a tert-butyl. hydroperoxide (tbOOH) induced oxidative injury model. Methods and results: The conditioned medium of ADSCs (ADSC-CM) was harvested and tested for antioxidant action. ADSC-CM had an antioxidant effect as potent as 100 mu M ascorbic acid and various antioxidant proteins were detected in ADSC-CM by proteomic analysis. Morphological change and cell survival assay revealed that incubation with ADSC-CM aided HDFs to resist free radicals induced by tbOOH. In addition, activities of superoxide dismutase and glutathione peroxidase were enhanced in the ADSC-CM treated HDFs which confirmed the study hypothesis that ADSCs protect HDFs through antioxidant action. In a cell cycle analysis, ADSC-CM treatment reversed the apoptotic cell death induced by tbOOH and caused a decrease of sub-G1 cells with respect to untreated cells. The anti-apoptotic effect of ADSC-CM was also reproduced by caspase-3 activity assay. Conclusion: These results suggest that ADSCs have potent antioxidant activity and protect HDFs from oxidative injury by decreasing apoptotic cells. Therefore, ADSCS and ADSC-CM are good candidates for control and prevention of skin damage from free radicals in various skin conditions. (c) 2007 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.