Corticotropin-releasing hormone and pro-opiomelanocortin gene expression in female monkeys with differences in sensitivity to stress
Corticotropin-releasing hormone and pro-opiomelanocortin gene expression in female monkeys with differences in sensitivity to stress
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DOI：
10.1159/000109877
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发表时间：
2007-01-01
影响因子：
4.1
通讯作者：
Bethea, Cynthia L.
中科院分区：
文献类型：
--
作者：
Centeno, Maria Luisa;Sanchez, Rachel L.;Bethea, Cynthia L.
背景/目的：在无应激雌性食蟹猴的脑组织中评估促肾上腺皮质激素释放激素（CRH）和阿片黑皮质素原（POMC）的表达，这些雌性食蟹猴先前被分类为与应激诱导的无排卵有关的高应激弹性（HSR）、中应激弹性（MSR）或应激敏感（SS）。方法：采用原位杂交和定量图像分析方法测量下丘脑室旁核（PVN）和丘脑中心正中束下复合体（CM-Sf）中编码CRH的mRNA。然后，对PVN中的CRH神经元进行免疫染色并测量免疫染色的面积。另外，对杏仁核中央核的CRH纤维进行免疫染色，并获得免疫染色的面积。最后，对下丘脑漏斗核中的 POMC mRNA 表达进行了表征。使用 ANOVA 和 Student-Newman-Keul (SNK) 事后比较对各组进行比较。结果：与 HSR 动物相比，MSR 和 SS 动物尾侧 PVN 中的 CRH mRNA 显着升高（p < 0.05，SNK）。与 HSR 组相比，MSR 和 SS 组的平均和总 CRH 阳性面积显着增加（p < 0.05，SNK）。与 HSR 组相比，MSR 和 SS 组的 CRH 体积也显着增加（p < 0.05，SNK）。在 CM-Sf 中，MSR 和 SS 组的平均 CRH 光密度显着高于 HSR 组（p < 0.05，SNK）。在杏仁核中央核中，SS 组的 CRH 纤维染色面积显着高于 MSR 或 HSR 组（p < 0.05，SNK）。下丘脑内侧基底层 POMC mRNA 表达在各组之间没有差异。结论：在压力下表现出生殖功能立即抑制的猕猴被认为是压力敏感的。这些动物的下丘脑和边缘结构中的 CRH 升高，这可能在应激开始时抑制下丘脑-性腺轴中发挥作用。版权所有 (C) 2007 S. Karger AG，巴塞尔。
Background/Aims: The expressions of corticotropin-releasing hormone (CRH) and pro-opiomelanocortin (POMC) were assessed in brain tissue collected from nonstressed female cynomolgus monkeys previously categorized as highly stress resilient (HSR), medium stress resilient (MSR), or stress sensitive (SS) with respect to stress-induced anovulation. Methods: In situ hybridization and quantitative image analysis was used to measure mRNAs coding for CRH in the hypothalamic paraventricular nucleus (PVN) and thalamic center median-subfascicular complex (CM-Sf). Then, CRH neurons in the PVN were immunostained and the area of immunostaining was measured. Also, CRH fibers were immunostained in the central nucleus of the amygdala and the area of immunostaining was obtained. Finally, POMC mRNA expression was characterized in the hypothalamic infundibular nucleus. The groups were compared with ANOVA and Student-Newman-Keul's (SNK) post hoc comparison. Results: CRH mRNA was significantly elevated in the caudal PVN in the MSR and SS animals compared to HSR animals (p < 0.05, SNK). There was a significant increase in average and total CRH-positive area in the MSR and SS groups compared to the HSR group (p < 0.05, SNK). There was also a significant increase in CRH volume in the MSR and SS groups compared to the HSR group (p < 0.05, SNK). In the CM-Sf, the average CRH optical density was significantly higher in the MSR and SS groups than in the HSR group (p < 0.05, SNK). In the central nucleus of the amygdala, the area of CRH fiber staining was significantly higher in the SS group than in the MSR or HSR groups (p < 0.05, SNK). There was no difference between the groups in POMC mRNA expression in the mediobasal hypothalamus. Conclusion: Macaques that exhibit immediate suppression of reproductive function upon stress are considered stress sensitive. These animals have elevated CRH in the hypothalamus and limbic structures, which may play a role in suppressing the hypothalamic-gonadal axis upon stress initiation. Copyright (C) 2007 S. Karger AG, Basel.