A simple technique to establish a long-term adenovirus mediated gene transfer to the heart of newborn mice.
A simple technique to establish a long-term adenovirus mediated gene transfer to the heart of newborn mice.
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DOI：
10.2174/187152909788488645
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发表时间：
2009-06
期刊：
影响因子：
--
通讯作者：
Ray PE
中科院分区：
文献类型：
--
作者：
Jerebtsova M;Ye X;Ray PE
以前使用不同技术的研究表明，腺病毒介导的基因转移到不同组织，包括肾脏，在新生小鼠中更有效。在这项研究中，我们报告了一种简单的技术，允许有效和长期的表达β-半乳糖苷酶（β-gal）在新生小鼠的心脏。新生和成年C57 BL 6/J小鼠经眶后静脉丛注射携带lac Z基因的重组腺病毒载体（rAd）（2 × 109个/g体重）。注射后7 d，在新生小鼠的心、肺、肠、肝、肾和脾中系统地观察到核周β-gal阳性染色。然而，只有心脏在初次注射后一年显示出β-gal的持续表达。相比之下，成年小鼠仅显示主要在肝脏中的显著但短暂的β-gal表达。总之，我们发现，单次眶后静脉注射可用于建立长期的腺病毒介导的基因转移到新生小鼠的心脏细胞。
Previous studies using different techniques have shown that adenoviral-mediated gene transfer to different tissues, including the kidney, is more efficient in neonatal mice. In this study, we report a simple technique that allows an efficient and long term expression of β-galactosidase (β-gal) in the heart of newborn mice. Newborn and adult C57BL6/J mice were subjected to a single retro-orbital venous plexus injection of recombinant adenoviral vector (rAd) (2 × 109 particles/g body weight) carrying the lac Z gene. Seven days after the injection, positive perinuclear β-gal staining was systematically observed in the heart, lung, intestine, liver, kidney and spleen of newborn mice. However, only the heart showed persistent expression of β-gal one year after the initial injection. In contrast, adult mice showed only significant but transient β-gal expression mainly in the liver. In summary, we have found that a single retro-orbital intravenous injection can be used to establish a long-term adenoviral-mediated gene transfer to cardiac cells of newborn mice.