IgE epitopes of intact and digested Ara h 1: A comparative study in humans and rats
IgE epitopes of intact and digested Ara h 1: A comparative study in humans and rats
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DOI：
10.1016/j.molimm.2012.04.002
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发表时间：
2012-07-01
影响因子：
3.6
通讯作者：
Roggen, E. L.
中科院分区：
文献类型：
--
作者：
Bogh, K. L.;Nielsen, H.;Roggen, E. L.
背景：过敏原表位的表征为了解蛋白质作为食物过敏原提供了有价值的信息。IgE抗原表位一般是构象性的，然而，对于已知通过胃肠道致敏的食物过敏原，线性表位被认为是非常重要的。目的：本研究的目的是鉴定完整和消化的Ara h 1的IgE特异性表位，并比较人和大鼠的表位模式。来自5名花生过敏患者和5只布朗挪威大鼠的血清被用于通过使用来自个体血清的多克隆IgE对噬菌体展示的随机七聚体肽库进行竞争性免疫筛选来识别完整和消化的Ara h 1特异性IgE表位。由此产生的肽序列被映射到表面上的一个三维结构的Ara h 1分子模拟epitopes使用基于计算机的algorithm.Results：患者以及大鼠被证明具有个人的IgE表位模式。所有的表位模拟物的构象，并发现成簇的Ara h 1分子的三个不同的领域。患者IgE鉴定出五个表位基序，迄今为止占了大部分洗脱的肽序列。完整的和消化的Ara h 1以及人和大鼠的表位模式相当相似。主要过敏原Ara h 1的个体特异性表位模式已被鉴定。IgE结合表位已被认为是食物过敏持续性和严重性的生物标志物，因此识别特定的表位模式或基序可能是预防、诊断和食物过敏的治疗。(C)2012爱思唯尔有限公司保留所有权利。
Background: Allergen epitope characterization provides valuable information useful for the understanding of proteins as food allergens. It is believed that IgE epitopes in general are conformational, nevertheless, for food allergens known to sensitize through the gastrointestinal tract linear epitopes have been suggested to be of great importance.Objective: The aim of this study was to identify IgE specific epitopes of intact and digested Ara h 1, and to compare epitope patterns between humans and rats.Methods: Sera from five peanut allergic patients and five Brown Norway rats were used to identify intact and digested Ara h 1-specific IgE epitopes by competitive immunoscreening of a phage-displayed random hepta-mer peptide library using polyclonal IgE from the individual sera. The resulting peptide sequences were mapped on the surface of a three-dimensional structure of the Ara h 1 molecule to mimic epitopes using a computer-based algorithm.Results: Patients as well as rats were shown to have individual IgE epitope patterns. All epitope mimics were conformational and found to cluster into three different areas of the Ara h 1 molecule. Five epitope motifs were identified by patient IgE, which by far accounted for most of the eluted peptide sequences. Epitope patterns were rather similar for both intact and digested Ara h 1 as well as for humans and rats.Conclusions: Individual patient specific epitope patterns have been identified for the major allergen Ara h 1.IgE binding epitopes have been suggested as biomarkers for persistency and severity of food allergy, wherefore recognition of particular epitope patterns or motifs could be a valuable tool for prevention, diagnosis, and treatment of food allergy. (C) 2012 Elsevier Ltd. All rights reserved.