Topical Dermal Application of Essential Oils Attenuates the Severity of Adjuvant Arthritis in Lewis Rats
Topical Dermal Application of Essential Oils Attenuates the Severity of Adjuvant Arthritis in Lewis Rats
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DOI：
10.1002/ptr.3509
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发表时间：
2012-01-01
影响因子：
7.2
通讯作者：
Moudgil, Kamal D.
中科院分区：
文献类型：
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作者：
Komeh-Nkrumah, Steva A.;Nanjundaiah, Siddaraju M.;Moudgil, Kamal D.
本研究旨在研究含有精油 (EO) 的软膏对 Lewis 大鼠佐剂性关节炎 (AA)（一种人类类风湿性关节炎 (RA) 实验模型）严重程度的影响，并确定其潜在机制。 AA 开始时，大鼠每天局部涂抹两次含有 20% EO 的软膏或安慰剂软膏。检测大鼠滑液（SF）和滑膜浸润细胞（SIC）的促炎细胞因子TNF-α和IL-1β。对后爪和皮肤进行组织学检查。测试了基质金属蛋白酶 (MMP) 和抗分枝杆菌热休克蛋白 65 (Bhsp65) 抗体的活性/水平。与对照组相比，用含有 EO 的软膏治疗的关节炎大鼠出现了较轻的临床关节炎，并且这种活性归因于 EO 而不是基础油。与对照组相比，EO治疗的关节炎大鼠的TNF-α和IL-1β水平以及SF和SIC裂解物中MMP的活性显着降低。然而，抗 Bhsp65 抗体的水平并未受到治疗的影响。因此，局部真皮递送含 EO 的软膏可通过抑制特定的炎症介质来下调 Lewis 大鼠 AA 的严重程度。此类药膏应在患有 RA 和其他关节炎疾病的患者中进行测试。版权所有 (C) 2011 John Wiley & Sons, Ltd.
This study was aimed at examining the effect of an ointment containing essential oils (EO) on the severity of adjuvant arthritis (AA), an experimental model of human rheumatoid arthritis (RA), in Lewis rats and to define the underlying mechanisms. At the onset of AA, the rats received topical application twice daily of an ointment containing 20% EO or placebo ointment. The synovial fluid (SF) and synovium-infiltrating cells (SIC) of rats were tested for pro-inflammatory cytokines TNF-alpha and IL-1 beta. The hind paws and skin were examined histologically. The activity/level of matrix metalloproteinases (MMPs) and anti-mycobacterial heat-shock protein 65 (Bhsp65) antibodies were tested. Arthritic rats treated with ointment containing EO developed less severe clinical arthritis compared with the controls, and this activity was attributable to EO and not to the carrier oil. The levels of TNF-alpha and IL-1 beta, and the activity of MMPs in SF and SIC-lysate were significantly reduced in EO-treated arthritic rats compared with the controls. However, the levels of anti-Bhsp65 antibodies were unaffected by treatment. Thus, topical dermal delivery of EO-containing ointment down-modulates the severity of AA in Lewis rats by inhibiting defined mediators of inflammation. Such ointments should be tested in patients with RA and other arthritic conditions. Copyright (C) 2011 John Wiley & Sons, Ltd.