Isolation of lineage-restricted neuronal precursors from multipotent neuroepithelial stem cells
Isolation of lineage-restricted neuronal precursors from multipotent neuroepithelial stem cells
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DOI：
10.1016/s0896-6273(00)80960-5
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发表时间：
1997-10-01
期刊：
影响因子：
16.2
通讯作者：
Rao, MS
中科院分区：
文献类型：
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作者：
MayerProschel, M;Kalyani, AJ;Rao, MS
我们已经鉴定了表达E-NCAM（高聚硅酸NCAM）并且在形态上不同于多能神经上皮（NEP）细胞的神经元限制性前体（NRP）细胞（Kalyani等人，1997）和脊髓神经胶质祖细胞（Rao和Mayer-Proschel，1997）。NRP细胞在成纤维细胞生长因子（FGF）和神经营养因子-3（NT-3）存在下在多次传代中自我更新，并且在视黄酸存在和FGF不存在下分化成有丝分裂后神经元。NRP细胞也可以由多能E10.5 NEP细胞产生。克隆分析表明，NRP细胞产生于产生其他限制性CNS前体的NEP祖细胞。NEP衍生的NRPs进行自我更新，并可以分化成多种神经元表型。因此，直接的线性关系之间存在多能NEP细胞和更有限的神经元前体细胞在体内存在于E13.5在脊髓。
We have identified a neuronal-restricted precursor (NRP) cell that expresses E-NCAM (high polysialic-acid NCAM) and is morphologically distinct from multipotent neuroepithelial (NEP) cells (Kalyani et al., 1997) and spinal glial progenitors (Rao and Mayer-Proschel, 1997). NRP cells self renew over multiple passages in the presence of fibroblast growth factor (FGF) and neurotrophin-3 (NT-3) and differentiate in the presence of retinoic acid and the absence of FGF into postmitotic neurons. NRP cells can also be generated from multipotent E10.5 NEP cells. Clonal analysis shows that NRP cells arise from a NEP progenitor that generates other restricted CNS precursors. The NEP-derived NRPs undergo self renewal and can differentiate into multiple neuronal phenotypes. Thus, a direct lineal relationship exists between multipotential NEP cells and more restricted neuronal precursor cells present in vivo at E13.5 in the spinal cord.