PRESENCE OF TROPOMYOSIN IN ADRENAL CHROMAFFIN CELLS AND ITS ASSOCIATION WITH CHROMAFFIN GRANULE MEMBRANES
PRESENCE OF TROPOMYOSIN IN ADRENAL CHROMAFFIN CELLS AND ITS ASSOCIATION WITH CHROMAFFIN GRANULE MEMBRANES
复制标题
DOI：
10.1016/0014-5793(85)80183-6
复制
发表时间：
1985-01-01
期刊：
影响因子：
3.5
通讯作者：
NORMAN, KM
中科院分区：
文献类型：
--
作者：
BURGOYNE, RD;NORMAN, KM
通过抗原肌球蛋白抗体对培养的嗜铬细胞进行免疫过氧化物酶染色，证明了肾上腺嗜铬细胞中存在原肌球蛋白。在免疫印迹中，抗体标记了 100000 × g 上清液和肾上腺髓质热稳定级分中的 Mr39000、36500 和 31000 的多肽。肾上腺髓质原肌球蛋白不与平滑肌原肌球蛋白共迁移。 39-kDa 和 31-kDa 多肽与脑上清液中抗原肌球蛋白标记的多肽共迁移。嗜铬颗粒膜具有由抗原肌球蛋白标记的 39-kDa 多肽。
The presence of tropomyosins in adrenal chromaffin cells was demonstrated by immunoperoxidase staining of cultured chromaffin cells by an anti‐tropomyosin antibody. In immunoblotting the antibody labelled polypeptides ofMr39000, 36500 and 31000 in 100000 ×gsupernatants and heat‐stable fractions of adrenal medulla. The adrenal medullary tropomyosins did not comigrate with smooth muscle tropomyosins. The 39‐kDa and 31‐kDa polypeptides comigrated with polypeptides labelled by anti‐tropomyosin in brain supernatants. Chromaffin granule membranes possessed a 39‐kDa polypeptide that was labelled by anti‐tropomyosin.