Acrosome-reacted mouse spermatozoa recovered from the perivitelline space can fertilize other eggs
Acrosome-reacted mouse spermatozoa recovered from the perivitelline space can fertilize other eggs
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DOI：
10.1073/pnas.1116965108
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发表时间：
2011-12-13
影响因子：
11.1
通讯作者：
Yanagimachi, Ryuzo
中科院分区：
文献类型：
--
作者：
Inoue, Naokazu;Satouh, Yuhkoh;Yanagimachi, Ryuzo
许多研究者认为，在到达卵透明带（ZP）表面之前已经启动顶体反应（AR）的精子不能结合和穿透ZP。最近的一项研究表明，大多数小鼠精子在接触ZP之前启动AR。我们发现，顶体反应的精子收集的Cd 9-null小鼠（其卵质膜不能与精子融合）的卵周间隙能够通过两个卵丘和ZP的WT小鼠卵，并产生活的后代。这意味着我们使用的精子至少有两次通过ZP的能力。显然，一些在与ZP接触之前很久就经历了AR的精子仍然能够穿过ZP和受精卵。因此，顶体反应的精子不能与ZP结合并失去其抑制能力的观点必须重新考虑。
Many investigators maintain that spermatozoa that have initiated the acrosome reaction (AR) before reaching the surface of the egg's zona pellucida (ZP) are unable to bind and penetrate the ZP. A recent study has revealed that most fertilizing mouse spermatozoa initiate the AR before contacting the ZP. We found that acrosome-reacted spermatozoa collected from the perivitelline space of Cd9-null mice (whose egg plasma membranes are incapable of fusing with spermatozoa) were able to pass through both the cumulus and ZP of WT mouse eggs and produced live offspring. This means that the spermatozoa we used had the ability to pass through the ZP at least twice. Apparently, some spermatozoa that had undergone the AR long before contact with the ZP remained capable of crossing the ZP and fertilizing eggs. Thus, the concept that acrosome-reacted spermatozoa are unable to bind to the ZP and have lost their fertilizing capacity must be reconsidered.