Patient-derived orthotopic xenograft (PDOX) mouse model of adult rhabdomyosarcoma invades and recurs after resection in contrast to the subcutaneous ectopic model
Patient-derived orthotopic xenograft (PDOX) mouse model of adult rhabdomyosarcoma invades and recurs after resection in contrast to the subcutaneous ectopic model
复制标题
DOI：
10.1080/15384101.2016.1252885
复制
发表时间：
2017-01-01
期刊：
影响因子：
4.3
通讯作者：
Hoffman, Robert M.
中科院分区：
文献类型：
--
作者：
Igarashi, Kentaro;Kawaguchi, Kei;Hoffman, Robert M.
横纹肌肉瘤是一种罕见的间叶性肿瘤。本研究的目的是开发RMS的患者来源的原位异种移植（PDOX）小鼠模型，并将PDOX模型与皮下（s.c.）移植模型将来自横纹肌的患者RMS原位生长在裸鼠的右股二头肌和右四头肌中以建立PDOX模型，以及皮下生长以建立皮下移植模型。模型PDOX肿瘤以与s.c.肿瘤的手术切除后复发仅发生在PDOX肿瘤，而不是在s.c.模型在组织学上，仅PDOX模型显示为侵入性的。总之，这些结果表明成人RMS的PDOX模型是恶性的，皮下模型是良性的。这些结果强调了适当的肿瘤微环境对于小鼠模型中肿瘤的患者样行为是必要的。
Rhabdomyosarcoma (RMS) is a rare mesenchymal tumor. The aim of the present study was to develop a patient-derived orthotopic xenograft (PDOX) mouse model of RMS and compare the PDOX model to a subcutaneous (s.c.)-transplant model. A patient RMS from a striated muscle was grown orthotopically in the right biceps femoris muscle and right quadriceps muscle of nude mice to establish a PDOX model, as well as under the skin to establish an s.c. model. PDOX tumors grew at a statistically-significant faster rate compared to the s.c. tumors. Recurrence after surgical resection occurred only in PDOX tumors, not in the s.c. model. Histologically, only the PDOX model was shown to be invasive. In conclusion, these results indicate that the PDOX model of adult RMS is malignant and the subcutaneous model is benign. These results emphasize that a proper tumor microenvironment is necessary for patient-like behavior of a tumor in a mouse model.