TUBULIN BOUND TO COLCHICINE FORMS POLYMERS DIFFERENT FROM MICROTUBULES
TUBULIN BOUND TO COLCHICINE FORMS POLYMERS DIFFERENT FROM MICROTUBULES
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DOI：
10.1073/pnas.79.22.6753
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
TIMASHEFF, SN
中科院分区：
文献类型：
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作者：
ANDREU, JM;TIMASHEFF, SN
纯化的微管蛋白-秋水仙碱复合物在促进从纯化的微管蛋白组装的相同条件下进行体外聚合。对临界浓度的需要、反应的明显自由能变化以及二价阳离子和核苷酸结合的影响表明，这些相互作用与微管形成过程中涉及的相互作用相似。形成的大聚合物不是微管，这表明抗有丝分裂药物的作用模式可能是微管分子之间不正确的键合几何形状的产生。[使用小牛脑微管蛋白]。
The purified tubulin-colchicine complex undergoes in vitro polymerization under the same conditions that promote the assembly of microtubules from purified tubulin. The need for a critical concentration, the apparent free energy change of the reaction and the effects of divalent cations and nucleotide binding indicate interactions similar to those involved in microtubule formation. The large polymers formed are not microtubules, suggesting that the mode of action of the antimitotic drug may be the production of an incorrect bonding geometry between tubulin molecules. [Calf brain tubulin was used.].