Dissection of the Blue-Light-Dependent Signal-Transduction Pathway Involved in Gametic Differentiation of Chlamydomonas reinhardtii
Dissection of the Blue-Light-Dependent Signal-Transduction Pathway Involved in Gametic Differentiation of Chlamydomonas reinhardtii
复制标题
莱茵衣藻配子分化中蓝光依赖性信号转导途径的剖析
作者：
J. Pan;M. Haring;C. Beck
绿藻莱茵衣藻的配子发生可以被视为一个由氮剥夺和蓝光环境因素控制的两步过程。当细胞保存在黑暗中时，氮剥夺会诱导配子发生的启动，导致配子前无法交配。为了完成配子分化，需要光。用药理学化合物处理前配子以影响光依赖性向成熟配子的转化。研究发现二丁酰环 3°5° 腺苷一磷酸、罂粟碱和染料木黄酮在光照下可抑制配子发生的进展。在黑暗中用十字孢菌素或罂粟碱处理配子前，导致它们转化为成熟配子。显然，罂粟碱在黑暗和光照下有不同的作用；星形孢菌素的作用表明，蛋白激酶 C 样成分会抑制前配子向配子的转化，这种阻碍通常可以通过光照缓解。蛋白激酶 C、N-庚基-5-氯-1-萘磺酰胺、N-(6-苯基己基)-5-氯-1-萘磺酰胺和佛波醇酯佛波醇-12-肉豆蔻酸酯 13-乙酸酯的激活剂在光下抑制配子发生的观察结果证实了这一假设。金雀异黄素和二丁酰环 3[prime]5[prime] 腺苷单磷酸能够抑制星形孢菌素治疗引起的暗激活，表明它们的靶标在“蛋白激酶 C 样”激酶的下游发挥作用。令人惊讶的是，十字孢碱和罂粟碱在黑暗中协同作用激活前配子。
Gametogenesis of the green alga Chlamydomonas reinhardtii may be viewed as a two-step process that is controlled by the environmental cues of nitrogen deprivation and blue light. Initiation of gametogenesis is induced by nitrogen deprivation, resulting in mating-incompetent pregametes, when cells are kept in the dark. For the completion of gametic differentiation light is required. Pregametes were treated with pharmacological compounds to influence the light-dependent conversion to mature gametes. Dibutyryl-cyclic 3[prime]5[prime] adenosinemonophosphate, papaverine, and genistein were found to inhibit the progression of gametogenesis in the light. Treatment of pregametes in the dark with either staurosporine or papaverine resulted in their conversion to mature gametes. Apparently, papaverine has different effects in the dark and in the light; the effect of staurosporine suggested that a protein kinase C-like component inhibits the conversion of pregametes to gametes, a block that normally is relieved by illumination. This hypothesis was corroborated by the observation that activators of protein kinase C, N-heptyl-5-chloro-1-naphthalenesulfonamide, N- (6-phenylhexyl)-5-chloro-1-naphthalenesulfonamide, and the phorbolester phorbol-12-myristate 13-acetate inhibited gametogenesis in the light. Genistein and dibutyryl-cyclic 3[prime]5[prime] adenosinemonophosphate were able to inhibit the dark activation caused by staurosporine treatment, suggesting that their targets work downstream from the “protein kinase C-like” kinase. Surprisingly, staurosporine and papaverine worked synergystically on the activation of pregametes in the dark.
来自衣藻鞭毛膜的性凝集素。
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Adair,WS;Monk,BC;Cohen,R;Hwang,C;Goodenough,UW
通讯作者：
Goodenough,UW