TerF, the sixth identified replication arrest site in Escherichia coli, is located within the rcsC gene.
TerF, the sixth identified replication arrest site in Escherichia coli, is located within the rcsC gene.
复制标题
TerF 是大肠杆菌中第六个确定的复制停滞位点，位于 rcsC 基因内。
DOI：
10.1128/jb.174.23.7854-7858.1992
复制
发表时间：
1992
影响因子：
3.2
通讯作者：
Hill,TM
中科院分区：
文献类型：
--
作者：
Sharma,B;Hill,TM
我们报告了第六个复制阻止位点TerF的存在，它位于rcsC基因的编码序列中，rcsC基因是胶囊生物合成的负调节因子。TerF位点的定位允许rcsC基因的转录，但阻止DNA在末端到起点方向的复制。我们的研究结果表明，TerF位点在染色体和质粒环境中都具有功能，并且与染色体TerB位点相比，Tus-TerF蛋白- dna复合物的稳定性更接近于质粒R6K Ter位点。
We report the existence of a sixth replication arrest site, TerF, that is located within the coding sequences of the rcsC gene, a negative regulator of capsule biosynthesis. The TerF site is oriented to allow transcription of the rcsC gene but prevent DNA replication in the terminus-to-origin direction. Our results demonstrate that the TerF site is functional in both chromosomal and plasmid environments and that the stability of the Tus-TerF protein-DNA complex more closely resembles the plasmid R6K Ter sites than the chromosomal TerB site.