Placental and cord blood brain derived neurotrophic factor levels are decreased in nondiabetic macrosomia
Placental and cord blood brain derived neurotrophic factor levels are decreased in nondiabetic macrosomia
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DOI：
10.1007/s00404-017-4414-9
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发表时间：
2017-08-01
影响因子：
2.6
通讯作者：
Yang, Xin-Jun
中科院分区：
文献类型：
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作者：
Cai, Qian-Ying;Zhang, Heng-Xin;Yang, Xin-Jun
为探讨非糖尿病巨大儿胎盘脑源性神经营养因子（BDNF）基因表达水平和脐血BDNF水平与巨大儿的关系，对58例非糖尿病巨大儿和59例正常出生体重儿进行了病例对照研究。数据来源于访谈和我院数据库。使用定量实时聚合酶链反应（n = 117）定量胎盘组织中的BDNF基因表达。采用酶联免疫吸附法检测90例新生儿脐血BDNF水平。多因素Logistic回归模型分析了BDNF水平与巨大儿的关系，非糖尿病巨大儿的胎盘BDNF基因表达（P = 0.026）和脐带血BDNF（P = 0.008）低于正常出生体重对照组。经阴道分娩的巨大儿脐带血BDNF显著低于对照组（P = 0.014），但巨大儿经阴道分娩与剖宫产分娩的脐带血BDNF无差异。正常新生儿脐血BDNF水平与胎龄呈正相关（r = 0.496，P < 0.001），巨大儿脐血BDNF水平与胎龄无相关性。总组中脐血BDNF与胎盘BDNF相对表达量呈正相关（r（s）= 0.245，P = 0.02）。较高的脐带血BDNF水平与非糖尿病巨大儿的保护作用独立相关（校正比值比0.992; 95%可信区间0.986-0.998）。与正常出生体重的新生儿相比，非糖尿病巨大儿胎盘BDNF基因表达和脐带血BDNF均下调。脐带BDNF可能部分来源于胎盘分泌的BDNF。较高的脐带血浆BDNF水平可防止非糖尿病巨大儿。
To measure levels of placental brain derived neurotrophic factor (BDNF) gene expression and umbilical cord blood BDNF in neonates with nondiabetic macrosomia and determine associations between these levels and macrosomia.This case-control study included 58 nondiabetic macrosomic and 59 normal birth weight mother-infant pairs. Data were collected from interviews and our hospital's database. BDNF gene expression was quantified in placental tissues using quantitative real-time polymerase chain reaction (n = 117). Umbilical cord blood BDNF levels were measured by enzyme-linked immunosorbent assay (n = 90). Multivariate logistic regression models were used to evaluate associations between BDNF levels and macrosomia.Placental BDNF gene expression (P = 0.026) and cord blood BDNF (P = 0.008) were lower in neonates with nondiabetic macrosomia than in normal birth weight controls. Cord blood BDNF was significantly lower in vaginally delivered macrosomic neonates than vaginally delivered controls (P = 0.014), but cord BDNF did not differ between vaginal and cesarean section delivery modes in macrosomic neonates. Cord blood BDNF was positively associated with gestational age in control neonates (r = 0.496, P < 0.001), but not in macrosomic neonates. Cord blood BDNF was positively associated with placental BDNF relative expression (r (s) = 0.245, P = 0.02) in the total group. Higher cord blood BDNF levels were independently associated with protection against nondiabetic macrosomia (adjusted odds ratio 0.992; 95% confidence interval 0.986-0.998).Both placental BDNF gene expression and cord blood BDNF were downregulated in neonates with nondiabetic macrosomia compared with normal birth weight neonates. Cord BDNF may partly derive from BDNF secreted by the placenta. Higher cord plasma BDNF levels protected against nondiabetic macrosomia.