Glucose Transporter 2 and 4 Are Involved in Glucose Supply during Pulpal Wound Healing after Pulpotomy with Mineral Trioxide Aggregate in Rat Molars
Glucose Transporter 2 and 4 Are Involved in Glucose Supply during Pulpal Wound Healing after Pulpotomy with Mineral Trioxide Aggregate in Rat Molars
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葡萄糖转运蛋白 2 和 4 参与大鼠磨牙中三氧化二矿物质聚集体活髓切开术后牙髓伤口愈合过程中的葡萄糖供应
DOI：
10.1016/j.joen.2019.10.003
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发表时间：
2020
影响因子：
4.2
通讯作者：
Noiri Yuichiro
中科院分区：
文献类型：
--
作者：
Tohma Aiko;Ohkura Naoto;Yoshiba Kunihiko;Takeuchi Ryosuke;Yoshiba Nagako;Edanami Naoki;Shirakashi Mari;Ibn Belal Razi Saifullah;Ohshima Hayato;Noiri Yuichiro
盖髓材料是一种能使牙髓损伤愈合并形成一层修复性牙本质的材料。治疗受伤部位需要葡萄糖。葡萄糖由葡萄糖转运蛋白（Glut）2和Glut 4转运，这两种跨膜蛋白充当守门人。我们假设葡萄糖通过Glut 2/Glut 4的转运可能有助于在伤口愈合过程中产生牙本质桥。因此，我们探讨Glut 2和Glut 4的表达在修复性牙本质形成后，矿物三氧化物聚合capping.MethodsThe左上第一磨牙8周龄Wistar大鼠进行活髓切断术与矿物三氧化物聚合。治疗后1、3、5、7和14天，通过免疫组织化学染色分析Glut 2、Glut 4、巢蛋白（成牙本质细胞标记物）和抗内皮细胞抗原1（RECA-1;内皮细胞标记物）的定位和共定位。采用实时荧光定量聚合酶链反应（PCR）技术，检测Glut 2、Glut 4、胰岛素样生长因子1受体（IGF-1 R）和nestin基因在正常对照牙的成牙本质细胞和内皮细胞中的表达。活髓切断后3天，检测到Glut 2和Glut 4阳性细胞;活髓切断后7天，Glut 2和Glut 4的免疫反应仅限于新分化的成牙本质细胞样细胞排列在修复性牙本质下。结论Glut 2和Glut 4参与了创伤愈合过程中葡萄糖转运的调控。这可能有助于发展新的活髓治疗深龋患者。
IntroductionPulp capping materials allow healing of injured pulp with a layer of reparative dentin. Glucose is needed to cure the injured area. Glucose is transported by glucose transporter (Glut) 2 and Glut4, which are transmembrane proteins that act as gatekeepers. We hypothesized that the transport of glucose via Glut2/Glut4 might contribute to the production of a dentin bridge during wound healing. Therefore, we explored Glut2 and Glut4 expression during reparative dentinogenesis after mineral trioxide aggregate capping.MethodsThe upper left first molar of 8-week-old Wistar rats underwent pulpotomy with mineral trioxide aggregate. At 1, 3, 5, 7, and 14 days after treatment, localization and colocalization of Glut2, Glut4, nestin (odontoblast marker), and antiendothelial cell antigen 1 (RECA-1; endothelial cell marker) were analyzed with immunohistochemical staining. Messenger RNA expression levels ofSlc2a2(encoding Glut2),Slc2a4(encoding Glut4),Igf-1r(encoding insulinlike growth factor 1 receptor), andnestinwere analyzed in the extracted teeth using real-time polymerase chain reaction.ResultsGlut2 and Glut4 were localized within odontoblasts and endothelial cells in normal control teeth. Three days after pulpotomy, Glut2- and Glut4-positive cells were detected; 7 days after pulpotomy, immunoreactivity for Glut2 and Glut4 was confined to newly differentiated odontoblastlike cells arranged beneath reparative dentin. Messenger RNA expression levels ofSlc2a2andSlc2a4were significantly up-regulated after pulpotomy.ConclusionsGlut2 and Glut4 regulate glucose transport during wound healing beneath the injured area. This may contribute to the development of new vital pulp therapy for patients with deep caries.