Hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) in Italy
Hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) in Italy
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DOI：
10.1186/1476-0711-8-22
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发表时间：
2009-01-01
影响因子：
5.7
通讯作者：
Stefani, Stefania
中科院分区：
文献类型：
--
作者：
Campanile, Floriana;Bongiorno, Dafne;Stefani, Stefania
我们研究的目的是追踪意大利医院相关的耐甲氧西林金黄色葡萄球菌 (HA-MRSA) 谱系的动态变化，将 17 年来当代分离株的基因型背景与 1980 年的早期 MRSA 菌株样本的基因型背景进行比较。总共 301 个非重复 MRSA 临床分离株，从 19 家意大利医院中回收 选择并分析 1990 和 2007 年的抗生素耐药性，通过 PFGE 和 SCCmec 分型，分为克隆类型，并使用多位点序列分型 (MLST) 进一步表征。还使用了 1980 年的 15 种早期 MRSA 菌株样本进行比较。最有趣的特征是，与 1990-1999 年期间 (29%) 相比，ST228-MRSA-I（以前的意大利克隆；PFGE E）最近在 2000-2007 年期间有所增加（57%），而且迄今为止其稳定性与高度流行的 ST247-MRSA-IA（以前的意大利克隆）的减少有关。 伊比利亚克隆人； PFGE A)，（1990 年至 1999 年为 23%，2000 年至 2007 年为 6%）。 ST1-MRSA-I（2 株中的 1 株携带 ccrA2B2）、ST8-MRSA-I（4 株）、ST15-MRSA-I（4 株中的 1 株携带 ccrA2B2）和 ST30-MRSA-I（5 株中的 2 株不携带 ccrAB 型和 ccrC）是 1980 年 MRSA 株中主要的最早 ST。 观察到对糖肽的敏感性水平：万古霉素 MIC >= 2 mg/L 的菌株从 19.4% 增加到 35.5%。 总之，我们描述了 1990 年至 2007 年医院中发生的 MRSA 克隆的交替以及糖肽 MIC 水平的增加，反映了世界范围内的趋势。我们记录了 1980 年分离株中 ST1、ST8、ST15 和 ST30 的检测情况；我们假设它们可能的潜伏期和外观与当前的 CA-MRSA 克隆一样。
The aim of our study was to trace the dynamic changes of hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) lineages in Italy, comparing the genotypic backgrounds of contemporary isolates over a period of 17 years, with those of a sample of early MRSA strains from 1980.In total, 301 non-repetitive MRSA clinical isolates, recovered from 19 Italian hospitals between 1990 and 2007 were selected and analyzed for their antibiotic resistance, typed by PFGE and SCCmec, grouped into clonal-types and further characterized using Multi Locus Sequence Typing (MLST). A sample of fifteen early MRSA strains from 1980 was also used for comparison.The most interesting feature was the recent increase of ST228-MRSA-I (formerly the Italian clone; PFGE E) over the period 2000-2007 (57%), when compared to the period 1990-1999 (29%), and its stability to date, associated with a decrease of the highly epidemic ST247-MRSA-IA (formerly the Iberian clone; PFGE A), (23% from 1990 to 1999, 6% from 2000 to 2007). ST1-MRSA-I (1 out of 2 strains carrying ccrA2B2), ST8-MRSA-I (4 strains), ST15-MRSA-I (1 out of 4 carrying ccrA2B2) and ST30-MRSA-I (2 out of 5 carrying no ccrAB-types and ccrC) were the predominant earliest STs among the MRSA strains in 1980.A temporal shift in the susceptibility levels to glycopeptides was observed: strains with vancomycin MIC of >= 2 mg/L increased from 19.4% to 35.5%.In conclusion, we describe the alternation of MRSA clones that occurred in hospitals from 1990 to 2007 and the increase of the glycopeptide MIC levels, reflecting a worldwide trend. We document the detection of ST1, ST8, ST15 and ST30 in the 1980 isolates; we hypothesize their possible latency and their appearance as the current CA-MRSA clones.