THE MELOLONTHA-MELOLONTHA ENTOMOPOXVIRUS (MMEPV) FUSOLIN IS RELATED TO THE FUSOLINS OF LEPIDOPTERAN EPVS AND TO THE 37K BACULOVIRUS GLYCOPROTEIN
THE MELOLONTHA-MELOLONTHA ENTOMOPOXVIRUS (MMEPV) FUSOLIN IS RELATED TO THE FUSOLINS OF LEPIDOPTERAN EPVS AND TO THE 37K BACULOVIRUS GLYCOPROTEIN
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DOI：
10.1006/viro.1995.1173
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发表时间：
1995-04-20
期刊：
影响因子：
3.7
通讯作者：
BERGOIN, M
中科院分区：
文献类型：
--
作者：
GAUTHIER, L;COUSSERANS, F;BERGOIN, M
我们克隆并测序了MmEPV基因组的一个1.7 kbp的DNA片段，该片段包含纺锤形内含物基因fusolin的主要多肽。该序列包含一个1203 nt的开放阅读框，能够编码45.8 kDa的多肽。13个n端氨基酸（aa）残基疏水，可作为信号肽。aa序列还含有13个半胱氨酸残基，这些残基很可能参与了准晶体的形成。该序列与两种鳞翅目动物的绒毛蛋白（chistoneura biennis EPV, CbEPV）和棉铃虫（Heliothis armigera EPV）具有显著的同源性，也与加利福尼亚Autographa californica和Orgyia pseudosugata杆状病毒的37K糖蛋白具有显著的同源性。MmEPV梭素与100K的MmEPV球蛋白没有同源性，与C6EPV和Amsacta moorei EPV球蛋白的110K多肽也没有同源性。这些数据经Western blot分析证实。用含有梭蛋白序列和上游调控区的质粒转染牛痘感染的哺乳动物细胞，可瞬时表达该基因。这表明牛痘转录机制能够转录梭蛋白基因。该梭素也通过重组杆状病毒在昆虫细胞中表达。(C) 1995学术出版社，Inc。
We have cloned and sequenced a 1.7-kbp DNA fragment of the MmEPV genome encompassing the major polypeptide of the spindle-shaped inclusions gene termed fusolin. The sequence contained a single open reading frame of 1203 nt capable of coding for a polypeptide of 45.8 kDa. The 13 N-terminal amino acid (aa) residues were hydrophobic and could act as a signal peptide. The aa sequence also contained 13 cysteine residues very likely involved in paracrystal formation. This sequence showed significant homologies with the fusolins of two lepidopteran EPVs, the Choristoneura biennis EPV (CbEPV) and the Heliothis armigera EPV, and also with the 37K glycoproteins of Autographa californica and Orgyia pseudotsugata baculoviruses. No homology was found between the MmEPV fusolin and the 100K MmEPV spherulin, nor with the 110K polypeptide of the C6EPV and Amsacta moorei EPV spheroidins. These data were confirmed by Western blot analysis. Transfection of vaccinia-infected mammalian cells with a plasmid encompassing the fusolin sequence plus the upstream regulatory region resulted in transient expression of the gene. This indicated that the vaccinia transcription machinery is able to transcribe the fusolin gene. The fusolin was also expressed in insect cells via a recombinant baculovirus. (C) 1995 Academic Press, Inc.