Prevalence and Antimicrobial Resistance of Salmonella Isolated from Cattle Feces in United States Feedlots in 2011
Prevalence and Antimicrobial Resistance of Salmonella Isolated from Cattle Feces in United States Feedlots in 2011
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DOI：
10.1089/fpd.2016.2128
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发表时间：
2016-09-01
影响因子：
2.8
通讯作者：
Fedorka-Cray, Paula J.
中科院分区：
文献类型：
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作者：
Dargatz, David A.;Kopral, Christine A.;Fedorka-Cray, Paula J.
本研究的目的是确定沙门氏菌的流行和特点。从美国饲养场的牛的粪便中分离出来。粪便样本收集自12个州的68个饲养场中的每一个饲养场的最多三个牛栏。样本包括来自畜栏地板的最多25个个体粪便块和来自相同畜栏地板的最多5个复合样本。沙门氏菌阳性样本的患病率分别为9.1%（460/5050）和11.3%（114/1009）的个人和复合样本。单个和混合样品的围栏水平沙门氏菌的患病率分别为35.6%（72/202）和22.8%（46/202）。饮食因素，包括棉籽壳，抗球虫药和抗微生物药物，与沙门氏菌分离的患病率差异有关。共鉴定出32个血清型，但6个血清型占分离株的69.1%（495/716）。近三分之二（64.7%，44/68）的饲养场至少有一个阳性样品。所有分离株进行了评价，对一组15种抗菌药物的敏感性。大多数分离株（74.4%，533/716）对该组中的所有抗菌药物敏感。当检测到耐药性时，最常见的是四环素（21.7%，155/716株）或磺胺异恶唑（12.4%，89/716株）。不到10%的分离株对测试条中的任何其他抗菌剂耐药。本研究的结果表明，沙门氏菌在个别粪便样品中的流行率低于10%，但沙门氏菌广泛分布于饲养场牛。此外，当饲养场牛中存在沙门氏菌时，除了四环素和磺胺异恶唑外，抗菌素耐药性的发生率较低。更多的研究表明，了解沙门氏菌的生态和抗生素耐药性，当存在时，在养牛业务。
The objective of this study was to determine the prevalence and characteristics of Salmonella spp. isolated from feces of cattle in feedlots in the United States. Fecal samples were collected from up to three pens of cattle in each of 68 feedlots in 12 states. Samples included up to 25 individual fecal pats from the pen floors and up to five composite samples from the floors of the same pens. The prevalence of Salmonella-positive samples was 9.1% (460/5050) and 11.3% (114/1009) for individual and composite samples, respectively. The prevalences of Salmonella at the pen level were 35.6% (72/202) and 22.8% (46/202) for individual and composite samples, respectively. Dietary factors, including inclusion of cottonseed hulls, coccidiostats, and antimicrobial drugs, were associated with differences in prevalence of Salmonella isolation. Overall, 32 serotypes of Salmonella were identified, but six serotypes accounted for 69.1% (495/716) of the isolates. Nearly two-thirds (64.7%, 44/68) of feedlots had at least one positive sample. All isolates were evaluated for susceptibility to a panel of 15 antimicrobial drugs. Most isolates (74.4%, 533/716) were susceptible to all antimicrobial drugs in the panel. When resistance was detected, it was most commonly to tetracycline (21.7%, 155/716 of isolates) or sulfisoxazole (12.4%, 89/716 of isolates). Less than 10% of the isolates were resistant to any other antimicrobials in the panel. The results of this study indicate that the prevalence of Salmonella in individual fecal samples was less than 10%, but that Salmonella is widely distributed among feedlot cattle. Furthermore, when Salmonella is present in feedlot cattle, there is a low occurrence of antimicrobial resistance with the exception of tetracycline and sulfisoxazole. More research is indicated to understand the ecology of Salmonella and antimicrobial resistance, when present, in cattle-feeding operations.