Intrauterine growth restriction and postnatal high-protein diet affect the kidneys in adult rats.
Intrauterine growth restriction and postnatal high-protein diet affect the kidneys in adult rats.
复制标题
宫内生长受限和产后高蛋白饮食会影响成年大鼠的肾脏。
作者：
Q. Shen;Hong Xu;Liming Wei;J. Chen;Hai
胎儿宫内生长受限（IUGR）与成年期的高血压和慢性肾脏疾病有关。IUGR后的产后营养过剩可能是糖尿病和心血管疾病发生的重要致病因素。本研究旨在确定IUGR和出生后高蛋白饮食对成年大鼠肾脏的影响。方法采用等热量蛋白限制法在孕鼠中诱导Sprague-Dawley大鼠宫内生长受限。将IUGR幼仔分为两组，即标准蛋白质饮食（IUGR组）和高蛋白质饮食（HP组）。应用比较蛋白质组学方法研究了正常成年大鼠和IUGR成年大鼠肾脏蛋白质表达谱的差异以及出生后高蛋白饮食对肾脏蛋白质表达谱的影响，结果表明：IUGR成年大鼠尿蛋白排泄量和血压均高于对照组，而HP饮食引起的高血压和蛋白尿比IUGR本身更严重。差异蛋白质组学分析发现12个蛋白质在IUGR组和对照组之间有显著差异表达，它们是转录调节因子和结构分子。HP和对照组之间的差异蛋白质组学分析发现13种蛋白质具有显著差异表达，并且主要参与机体代谢、氧化还原和凋亡调节。本研究为IUGR肾脏疾病的发病机制及产后营养过剩的影响提供了有价值的实验证据。
OBJECTIVEIntrauterine growth restriction (IUGR) is associated with hypertension and chronic kidney disease in adulthood. Postnatal overnutrition after IUGR may be of pathogenic importance for the development of diabetes and cardiovascular disease. This study was to identify the effects of IUGR and a postnatal high-protein diet on the kidneys in adult rats.METHODSIntrauterine growth restriction was induced in Sprague-Dawley rats by isocaloric protein restriction in pregnant dams. IUGR pups were divided into two groups that were a standard-protein diet (IUGR group) or a high-protein diet (HP group). A comparative proteomic method was used to study the differences of protein expression profiles between normal adult rats and adult rats with IUGR and the effects of a postnatal high-protein diet on the protein expression profiles of the kidneys.RESULTSThe IUGR adults had higher urinary excretion of protein and blood pressure than controls and the HP diet caused more severe hypertension and proteinuria than IUGR itself. The differential proteomic expression analysis found 12 proteins that had significantly differential expression between the IUGR and control groups, which were transcription regulators and structural molecules. The differential proteomic expression analysis between the HP and control groups found 13 proteins that had significantly differential expression and were involved primarily in body metabolism, oxidation reduction, and apoptosis regulation.CONCLUSIONAn HP diet intervention after IUGR worsens the severity of hypertension and proteinuria, and this study may provide valuable experimental evidence of proteins involved in the pathogenesis of kidney disease in IUGR and the effect of postnatal overnutrition.
DOI：
10.1097/01.asn.0000115334.65095.9b
发表时间：
2004-03-01
影响因子：
13.6
作者：
Thongboonkerd, V;Barati, MT;Klein, JB
通讯作者：
Klein, JB
影响因子：
4.2
作者：
Wu, GY;Bazer, FW;Spencer, TE
通讯作者：
Spencer, TE