The heat shock response in yeast: differential regulations and contributions of the Msn2p/Msn4p and Hsf1p regulons
The heat shock response in yeast: differential regulations and contributions of the Msn2p/Msn4p and Hsf1p regulons
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DOI：
10.1046/j.1365-2958.1999.01467.x
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发表时间：
1999-07-01
影响因子：
3.6
通讯作者：
Labarre, J
中科院分区：
文献类型：
--
作者：
Boy-Marcotte, E;Lagniel, G;Labarre, J
在酿酒酵母中，热休克转录因子Hsf1p和应激反应转录因子Msn2p和Msn4p被热休克激活。用[S-35]-蛋氨酸标记和双向凝胶电泳法比较突变株和野生型菌株，分析它们在热休克蛋白诱导中的各自贡献。在从25摄氏度到38摄氏度的转变中诱导的52个蛋白质中，有一半被发现依赖于Msn2p和/或Msn4p(主要包括抗氧化剂和参与碳代谢的酶)，而另一半(主要包括伴侣和相关蛋白质)依赖于Hsf1p。这两组蛋白质只有轻微的重叠。有三种蛋白质的诱导是独立于这些转录因子的，这表明有其他转录因子(S)的参与。Ras/cAMP/PKA信号通路cAMP对Msn2p/Msn4p调节子的诱导具有负性作用，但不影响Hsf1p调节子，因此，这两种转录因子的调控方式不同，控制着两组功能不同的蛋白质，提示在热休克细胞反应中有两种不同的生理作用。
The heat shock transcription factor Hsf1p and the stress-responsive transcription factors Msn2p and Msn4p are activated by heat shock in the yeast Saccharomyces cerevisiae. Their respective contributions to heat shock protein induction have been analysed by comparison of mutants and wild-type strains using [S-35]-methionine labelling and two-dimensional gel electrophoresis. Among 52 proteins induced by a shift from 25 degrees C to 38 degrees C, half of them were found to be dependent upon Msn2p and/or Msn4p (including mostly antioxidants and enzymes involved in carbon metabolism), while the other half (including mostly chaperones and associated proteins) were dependent upon Hsf1p. The two sets of proteins overlapped only slightly. Three proteins were induced independently of these transcription factors, suggesting the involvement of other transcription factor(s). The Ras/cAMP/PKA signalling pathway cAMP had a negative effect on the induction of the Msn2p/Msn4p regulon, but did not affect the Hsf1p regulon, Thus, the two types of transcription factor are regulated differently and control two sets of functionally distinct proteins, suggesting two different physiological roles in the heat shock cellular response.