The 60- to 90-kDa parietal cell autoantigen associated with autoimmune gastritis is a beta subunit of the gastric H+/K(+)-ATPase (proton pump).
The 60- to 90-kDa parietal cell autoantigen associated with autoimmune gastritis is a beta subunit of the gastric H+/K(+)-ATPase (proton pump).
复制标题
与自身免疫性胃炎相关的 60 至 90 kDa 壁细胞自身抗原是胃 H /K( )-ATP 酶（质子泵）的 β 亚基。
DOI：
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发表时间：
1990
影响因子：
11.1
通讯作者：
G. Baldwin
中科院分区：
文献类型：
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作者：
B. Toh;P. Gleeson;Richard J. SIMPSONt;Robert L. MORITZt;J. Callaghan;I. Goldkorn;Claerwen M. Jones;T. Martinelli;D. Humphris;J. Pettitt;Yumiko MORIt;Tohru MASUDAt;P. Sobieszczuk;J. Weinstock;T. Mantamadiotis;G. Baldwin
恶性贫血患者血清中的自身抗体除了识别胃H+/(+)-ATPase的阿尔法亚单位外，还识别一种丰富的胃微粒体糖蛋白，其表观MR为60,000-90,000。在这里，我们用免疫金电子显微镜将糖蛋白和胃H+/K(+)-ATPase的α亚基共定位于壁细胞的管泡膜上。此外，糖蛋白和α亚基被免疫共沉淀，并通过免疫亲和层析与抗糖蛋白单抗进行共沉淀。用番茄凝集素-琼脂糖柱层析纯化猪糖蛋白，并对纯化的糖蛋白中的5个胰蛋白酶多肽进行了部分测序。氨基酸序列与猪肾Na+/K(+)-ATPaseβ亚基序列有33%的相似性。因此，我们认为60-90 kDa的糖蛋白自身抗原是胃H+/K(+)-ATPase的β亚基，质子泵的α和β亚基是自身免疫性胃炎自身免疫的主要靶点。
Autoantibodies in the sera of patients with pernicious anemia recognize, in addition to the alpha subunit of the gastric H+/(+)-ATPase, an abundant gastric microsomal glycoprotein of apparent Mr 60,000-90,000. Herein we have colocalized the glycoprotein and the alpha subunit of the gastric H+/K(+)-ATPase to the tubulovesicular membranes of the parietal cell by immunogold electron microscopy. Moreover, the glycoprotein and the alpha subunit were coimmunoprecipitated, and copurified by immunoaffinity chromatography, with an anti-glycoprotein monoclonal antibody. The pig glycoprotein was purified by chromatography on tomato lectin-Sepharose, and five tryptic peptides from the purified glycoprotein were partially sequenced. The complete amino acid sequence, deduced from the nucleotide sequence of overlapping cDNA clones, showed 33% similarity to the sequence of the beta subunit of the pig kidney Na+/K(+)-ATPase. We therefore propose that the 60- to 90-kDa glycoprotein autoantigen is the beta subunit of the gastric H+/K(+)-ATPase and that the alpha and beta subunits of the proton pump are major targets for autoimmunization in autoimmune gastritis.