Bifidobacteria Enhance Antigen Sampling and Processing by Dendritic Cells in Pediatric Inflammatory Bowel Disease
Bifidobacteria Enhance Antigen Sampling and Processing by Dendritic Cells in Pediatric Inflammatory Bowel Disease
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DOI：
10.1097/mib.0000000000000389
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发表时间：
2015-07
影响因子：
4.9
通讯作者：
C. Strisciuglio;E. Miele;F. Giugliano;S. Vitale;M. Andreozzi;A. Vitale;M. Catania;A. Staiano;R. Troncone;C. Gianfrani
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文献类型：
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作者：
C. Strisciuglio;E. Miele;F. Giugliano;S. Vitale;M. Andreozzi;A. Vitale;M. Catania;A. Staiano;R. Troncone;C. Gianfrani
摘要：据报道，双歧杆菌可以减少炎症并有助于肠道内稳态。然而，这些细菌与肠道免疫系统之间的相互作用在很大程度上仍然未知。由于树突状细胞（DC）在免疫应答中发挥核心作用，我们在体外研究了双歧杆菌混合物（益生菌）对炎症性肠病儿童DC功能的影响。从克罗恩病（CD）、溃疡性结肠炎和非炎症性肠病对照（HC）患者的外周血单核细胞中获得DC，经益生菌预处理后，与大肠杆菌或dq -卵清蛋白（DQ-OVA）荧光染料结合颗粒孵育，以评估DC表型、抗原取样和处理。此外，收集细胞上清液，通过酶联免疫吸附法测定肿瘤坏死因子α、干扰素γ、白细胞介素17和白细胞介素10的产生。经益生菌培养的乳糜泻患儿DCs具有较高的细菌颗粒摄取和DQ-OVA加工；相比之下，溃疡性结肠炎和HC的DC无明显变化。此外，暴露于大肠杆菌颗粒后，在CD DC中观察到明显的肿瘤坏死因子α释放，而益生菌不影响这种促炎细胞因子的产生。综上所述，双歧杆菌显著改善了CD患者dc的抗原摄取和加工，而在溃疡性结肠炎和HC患者dc中，益生菌混合物没有观察到明显的作用。这种抗原采样和处理的改进可以部分解决儿童炎症性肠病肠道先天免疫功能受损，减少肠道内不受控制的微生物生长。
Abstract:Bifidobacteria have been reported to reduce inflammation and contribute to intestinal homeostasis. However, the interaction between these bacteria and the gut immune system remains largely unknown. Because of the central role played by dendritic cells (DCs) in immune responses, we examined in vitro the effects of a Bifidobacteria mixture (probiotic) on DC functionality from children with inflammatory bowel disease. DCs obtained from peripheral blood monocytes of patients with Crohn's disease (CD), ulcerative colitis, and noninflammatory bowel disease controls (HC) were incubated with fluorochrome-conjugated particles of Escherichia coli or DQ-Ovalbumin (DQ-OVA) after a pretreatment with the probiotic, to evaluate DC phenotype, antigen sampling and processing. Moreover, cell supernatants were collected to measure tumor necrosis factor alpha, interferon gamma, interleukin 17, and interleukin 10 production by enzyme-linked immunosorbent assay. DCs from CD children showed a higher bacteria particles uptake and DQ-OVA processing after incubation with the probiotic; in contrast, DC from both ulcerative colitis and HC showed no significant changes. Moreover, a marked tumor necrosis factor alpha release was observed in DC from CD after exposure to E. coli particles, whereas the probiotic did not affect the production of this proinflammatory cytokine. In conclusion, the Bifidobacteria significantly improved the antigen uptake and processing by DCs from patients with CD, which are known to present an impaired autophagic functionality, whereas, in DCs from ulcerative colitis and HC, no prominent effect of probiotic mixture was observed. This improvement of antigen sampling and processing could partially solve the impairment of intestinal innate immunity and reduce uncontrolled microorganism growth in the intestine of children with inflammatory bowel disease.