Neuropeptide Y in the olfactory system, forebrain and pituitary of the teleost, Clarias batrachus
Neuropeptide Y in the olfactory system, forebrain and pituitary of the teleost, Clarias batrachus
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DOI：
10.1016/j.jchemneu.2003.11.002
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发表时间：
2004-03-01
影响因子：
2.8
通讯作者：
Subhedar, N
中科院分区：
文献类型：
--
作者：
Gaikwad, A;Biju, KC;Subhedar, N
用免疫细胞化学方法研究了神经肽Y（NPY）样免疫反应阳性物质在胡子鲶前脑的分布。在嗅感受器神经元（ORN）中观察到明显的免疫反应性，它们在嗅神经中的投射，嗅神经层在球周围的神经束和内侧嗅束中延伸到端脑叶。嗅觉器官的消融导致嗅球的嗅神经层和内侧嗅束的神经束中的免疫反应性丧失。这一证据表明，神经肽Y可能作为一种神经递质在ORN的和传达化学感觉信息的嗅球，也到端脑的球外投射。此外，在嗅球中还发现了串珠状的免疫反应纤维网络，这些纤维对消融实验没有反应。这些纤维可能代表了球的离心神经支配。在终神经的部分神经节细胞中发现强免疫反应。免疫反应纤维和终末野广泛分布于端脑。脚内核的几个神经元呈中度免疫反应，和一个小的人口在视前核神经元室周也被标记。免疫反应终末领域是特别明显的视前区，结节区，脑室周围区周围的第三脑室和下叶。NPY免疫反应阳性细胞和纤维分布于垂体各叶。本研究结果描述了NPY在C.与其他硬骨鱼类的免疫反应模式一致。然而，神经肽Y在蝙蝠嗅觉系统中是一个新的功能，表明肽在处理化学感觉信息中的作用。(C)2003 Elsevier B.V.保留所有权利。
Distribution of neuropeptide Y (NPY)-like immunoreactivity in the forebrain of catfish Clarias batrachus was examined with immunocytochemistry. Conspicuous immunoreactivity was seen in the olfactory receptor neurons (ORNs), their projections in the olfactory nerve, fascicles of the olfactory nerve layer in the periphery of bulb and in the medial olfactory tracts as they extend to the telencephalic lobes. Ablation of the olfactory organ resulted in loss of immunoreactivity in the olfactory nerve layer of the bulb and also in the fascicles of the medial olfactory tracts. This evidence suggests that NPY may serve as a neurotransmitter in the ORN's and convey chemosensory information to the olfactory bulb, and also to the telencephalon over the extrabulbar projections. In addition, network of beaded immunoreactive fibers was noticed throughout the olfactory bulb, which did not respond to ablation experiment. These fibers may represent centrifugal innervation of the bulb. Strong immunoreactivity was encountered in some ganglion cells of nervus terminalis. Immunoreactive fibers and terminal fields were widely distributed in the telencephalon. Several neurons of nucleus entopeduncularis were moderately immunoreactive; and a small population of neurons in nucleus preopticus periventricularis was also labeled. Immunoreactive terminal fields were particularly conspicuous in the preoptic, the tuberal areas, and the periventricular zone around the third ventricle and inferior lobes. NPY immunoreactive cells and fibers were detected in all the lobes of the pituitary gland. Present results describing the localization of NPY in the forebrain of C. batrachus are in concurrence with the pattern of the immunoreactivity encountered in other teleosts. However, NPY in olfactory system of C batrachus is a novel feature that suggests a role for the peptide in processing of chemosensory information. (C) 2003 Elsevier B.V. All rights reserved.