Controlled translocation of DNA through nanopores in carbon nano-, silicon-nitride- and lipid-coated membranes.
Controlled translocation of DNA through nanopores in carbon nano-, silicon-nitride- and lipid-coated membranes.
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DOI：
10.1039/c4an02319f
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发表时间：
2015-06
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通讯作者：
A. Sischka;Lukas Galla;Andreas J Meyer;A. Spiering;Sebastian Knust;Michael Mayer;A. Hall;A. Beyer;P. Reimann;A. Gölzhäuser;D. Anselmetti
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作者：
A. Sischka;Lukas Galla;Andreas J Meyer;A. Spiering;Sebastian Knust;Michael Mayer;A. Hall;A. Beyer;P. Reimann;A. Gölzhäuser;D. Anselmetti
我们从实验和理论上研究了带电聚合物穿过固态纳米孔时的易位力，并发现纳米孔直径和纳米膜材料化学有明显的依赖性。为此，我们使用了专用的光学镊子力力学，能够探测负电荷双链DNA在氦离子钻孔纳米孔中的插入。我们发现纳米孔的直径和膜材料本身对通过纳米孔的电渗透流量有显著影响，从而对穿线力有显著影响。与裸氮化硅膜相比，DNA通过3纳米薄碳纳米膜和脂质双层纳米孔的穿线力分别增加了15%和85%。我们最近开发的理论模型定量地描述了这一发现，该模型还包括对易位DNA分子的水动力滑动效应和对膜厚度的力依赖。文中提出的额外测量结果进一步支持了我们的模型。
We investigated experimentally and theoretically the translocation forces when a charged polymer is threaded through a solid-state nanopore and found distinct dependencies on the nanopore diameter as well as on the nano membrane material chemistry. For this purpose we utilized dedicated optical tweezers force mechanics capable of probing the insertion of negatively charged double-stranded DNA inside a helium-ion drilled nanopore. We found that both the diameter of the nanopore and the membrane material itself have significant influences on the electroosmotic flow through the nanopore and thus on the threading force. Compared to a bare silicon-nitride membrane, the threading of DNA through only 3 nm thin carbon nano membranes as well as lipid bilayer-coated nanopores increased the threading force by 15% or 85%, respectively. This finding was quantitatively described by our recently developed theoretical model that also incorporates hydrodynamic slip effects on the translocating DNA molecule and the force dependence on the membrane thickness. The additional measurements presented in this paper further support our model.