Studying the stereostructures of biomolecules and their analogs by vibrational circular dichroism.
Studying the stereostructures of biomolecules and their analogs by vibrational circular dichroism.
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通过振动圆二色性研究生物分子及其类似物的立体结构。
DOI：
10.1038/pj.2016.61
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Taniguchi;T.;Hongen;T.;Monde;K.
生物大分子的功能在很大程度上取决于它们的立体结构（即构型和构象）。因此，详细了解它们的结构特性将有助于调节人工大分子的功能。然而，由于缺乏合适的分析技术，研究溶液状态下分子的立体结构通常是困难的。振动圆二色性（VCD）光谱，测量圆二色性在红外区域，表现出高灵敏度对分子立体结构。本文首先讨论了一种基于VCD理论计算的从小分子到大分子立体结构的解析方法。本文的其余部分是专门的VCD激子手性方法，一种新的方法，最近开发的作者解释VCD数据通过观察VCD偶联在C= O伸缩区域，各种生物分子，如肽，碳水化合物，聚酯和脂质的应用。
The functions of biomacromolecules are largely governed by their stereostructure (ie, configuration and conformation). Therefore, detailed understanding of their structural properties should help in regulating the functions of artificial macromolecules. However, studying the stereostructure of a molecule in the solution state is typically difficult due to the lack of suitable analytical techniques. Vibrational circular dichroism (VCD) spectroscopy, which measures circular dichroism in the infrared region, exhibits high sensitivity toward molecular stereostructures. In this paper, we first discuss a method for the elucidation of the stereostructures of small to large molecules based on theoretical calculations of VCD. The rest of the paper is dedicated to the applications of a VCD exciton chirality method, a novel approach recently developed by the authors to interpret VCD data by observing a VCD couplet in the C= O stretching region, to various biomolecules such as peptides, carbohydrates, polyesters and lipids.