Characterization of Multiple Helicobacter bizzozeronii Isolates From a Finnish Patient With Severe Dyspeptic Symptoms and Chronic Active Gastritis
Characterization of Multiple Helicobacter bizzozeronii Isolates From a Finnish Patient With Severe Dyspeptic Symptoms and Chronic Active Gastritis
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DOI：
10.1111/j.1523-5378.2009.00730.x
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发表时间：
2010-02-01
期刊：
影响因子：
4.4
通讯作者：
Hanninen, Marja-Liisa
中科院分区：
文献类型：
--
作者：
Kivisto, Rauni;Linros, Juri;Hanninen, Marja-Liisa
背景：幽门螺杆菌是人类胃炎和消化性溃疡的主要病因。在少数有上消化道症状的患者中，在胃活检中观察到长而紧密盘绕的螺旋细菌，暂时命名为“海尔曼螺杆菌”。这些细菌是非常挑剔的，只有一个以前的研究已经成功地获得了一个分离在体外。来自芬兰患者的胃粘膜的“heilmannii”，该患者表现出严重的消化不良症状。分离物的特点是测试脲酶和过氧化氢酶活性，通过使用光学和电子显微镜，并通过部分16S rRNA和ureAB基因测序。采用单酶扩增片段长度多态性（sAFLP）技术对分离株进行遗传多样性分析。结果：我们从不同的胃活检标本中获得了15株分离株，其中3株在患者治疗失败后获得。分离株经鉴定为bizzozeronii螺杆菌。根除治疗是不成功的，最有可能是由于高水平的耐药性甲硝唑。同一种H.结论：bizzozeronii螺杆菌仍是唯一的“H.从人胃粘膜分离的“Heilmannii”种，虽然在“H.在基于PCR的筛选研究中，H的相关性bizzozeronii和其他潜在的人畜共患胃螺杆菌属。在人类疾病中的作用仍有待确定。
Background: Helicobacter pylori is the primary cause of gastritis and peptic ulceration in humans. In a minority of patients with upper gastrointestinal symptoms, long tightly coiled spiral bacteria, provisionally named "Helicobacter heilmannii," are observed in gastric biopsies. These bacteria are extremely fastidious and only one previous study has succeeded in obtaining an isolate in vitro.Materials and Methods: We used two different selective media to isolate "H. heilmannii" from the gastric mucosa of a Finnish patient presenting with severe dyspeptic symptoms. The isolates were characterized by testing for urease and catalase activity, by using light and electron microscopy, and by sequencing of the partial 16S rRNA and ureAB genes. Single-enzyme amplified fragment length polymorphism (sAFLP) was used to analyze the genetic diversity among the isolates.Results: We obtained 15 isolates from different gastric biopsies prior and three after unsuccessful treatment of the patient. The isolates were identified as Helicobacter bizzozeronii. Eradication therapy was unsuccessful most probably due to high level of resistance to metronidazole. Persistent colonization by the same H. bizzozeronii clone was confirmed by sAFLP, however, small differences between the profiles suggested long-term colonization of the patient.Conclusions: Helicobacter bizzozeronii remains the only "H. heilmannii" species isolated from human gastric mucosa although it has been an infrequent observation among "H. heilmannii"-infected patients in PCR-based screening studies. The relevance of H. bizzozeronii and other potentially zoonotic gastric Helicobacter spp. in human disease remains to be determined.