Significant correlation between refractive index and activity of mitochondria: single mitochondrion study
Significant correlation between refractive index and activity of mitochondria: single mitochondrion study
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DOI：
10.1364/boe.6.000859
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发表时间：
2015-01-01
影响因子：
3.4
通讯作者：
Ohta, Yoshihiro
中科院分区：
文献类型：
--
作者：
Haseda, Keisuke;Kanematsu, Keita;Ohta, Yoshihiro
细胞内组分折射率（RI）的测量可以提供有关细胞结构和功能的有用信息。本研究报告，第一次，使用延迟调制差分干涉相差显微镜测定的RI的一个孤立的cadrons在等渗溶液中。该值为1.41 +/- 0.01，表明线粒体密集地填充有具有高RI的分子。此外，每个线粒体的RI与线粒体膜电位（线粒体活性的指数）显著相关。这些结果将提供有用的信息的结构和功能的基础上的细胞内分布的RI。(C)2015年美国光学学会。
Measurements of refractive indices (RIs) of intracellular components can provide useful information on the structure and function of cells. The present study reports, for the first time, determination of the RI of an isolated mitochondrion in isotonic solution using retardation-modulated differential interference contrast microscopy. The value was 1.41 +/- 0.01, indicating that mitochondria are densely packed with molecules having high RIs. Further, the RIs of each mitochondrion were significantly correlated with the mitochondrial membrane potential, an index of mitochondrial activity. These results will provide useful information on the structures and functions of cells based on the intracellular distribution of RIs. (C) 2015 Optical Society of America.