Structure of cDNA coding for rat platelet phospholipase A2.
Structure of cDNA coding for rat platelet phospholipase A2.
复制标题
编码大鼠血小板磷脂酶 A2 的 cDNA 结构。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
K. Inoue
中科院分区：
文献类型：
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作者：
M. Komada;I. Kudo;H. Mizushima;N. Kitamura;K. Inoue
从大鼠巨核细胞cDNA文库中分离出3个编码大鼠血小板磷脂酶A2及其同源蛋白的cDNA克隆，并进行了序列测定。一个（prPLA 2 -1）携带708个核苷酸长的插入物。其他的（prPLA 2 -2和-3）与克隆1在三个核苷酸上不同，并且具有748个核苷酸长的插入物。它们都含有编码146个氨基酸长的多肽的单个开放阅读框。基于推导的氨基酸序列，我们得出结论：prPLA 2 -1编码大鼠血小板磷脂酶A2。prPLA 2 -2和-3最可能编码与磷脂酶A2同源的蛋白质，具有两个氨基酸替换。一个典型的信号肽序列（21个氨基酸长），位于推导的结构的NH 2末端，紧随其后的是一个多肽，对应于成熟的酶，这表明大鼠血小板酶不表达为酶原形式。北方印迹分析显示，在大鼠巨核细胞、骨髓细胞、酪蛋白酸处理大鼠的腹膜细胞和脾细胞的多聚（A）+RNA组分中，存在一个900至1，100个核苷酸长的单一转录物。
Three cDNA clones coding for rat platelet phospholipase A2 and a homologous protein were isolated from a rat megakaryocyte cDNA library and sequenced. One (prPLA2-1) carries a 708 nucleotide long insert. The others (prPLA2-2 and -3) differ from clone 1 in three nucleotides and have a 748 nucleotide long insert. All contain a single open reading frame which encodes a 146 amino acid long polypeptide. Based on the deduced amino acid sequence, we concluded that prPLA2-1 encodes rat platelet phospholipase A2. prPLA2-2 and -3 most probably encode a protein homologous to phospholipase A2 with two amino acid replacements. A typical signal peptide sequence (21 amino acid long), located at the NH2 termini of the deduced structure, was immediately followed by a polypeptide which corresponds to the mature enzyme, suggesting that the rat platelet enzyme is not expressed as a proenzyme form. Northern blot analysis showed a single transcript, which is 900 to 1,100 nucleotides long, in the poly(A)+RNA fractions of rat megakaryocytes, bone marrow cells, peritoneal cells of caseinate-treated rats, and spleen cells.