Assessment of phospholipid deacylation-reacylation cycles by a stable isotope technique.
Assessment of phospholipid deacylation-reacylation cycles by a stable isotope technique.
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通过稳定同位素技术评估磷脂脱酰-再酰化循环。
DOI：
10.1016/0006-291x(87)90454-2
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发表时间：
1987
影响因子：
3.1
通讯作者：
Schmid,HH
中科院分区：
文献类型：
--
作者：
Kuwae,T;Schmid,PC;Schmid,HH
将小鼠腹腔渗出液细胞在含40%H218O的培养基中孵育1或2 h后，测定18 O掺入甘油磷脂中的情况。氢化脂肪酸甲酯的气相色谱-质谱分析表明，胆碱磷脂和磷脂酰肌醇中的18 O含量最高。通常存在于sn-1位的酰基所含的羰基18 O至少与那些在sn-2位的酰基一样多。考虑到18 O通过H2 18 O中酯水解产生的游离脂肪酸掺入的途径，某些腹膜渗出液细胞磷脂中的酰基周转率可能等于或超过20%/h。
Incorporation of 18 O into glycerophospholipids was determined after incubating mouse peritoneal exudate cells for 1 or 2 h in media containing 40% H 2 18 O. Gas chromatography-mass spectrometry of hydrogenated fatty acid methyl esters showed highest amounts of 18 O in choline phospholipids and phosphatidylinositol. Acyl groups generally present at the sn-1 position contained at least as much carbonyl 18 O as those at the sn-2 position. Considering the route of 18 O incorporation via free fatty acid derived through ester hydrolysis in H 2 18 O, acyl turnover in certain peritoneal exudate cell phospholipids may equal or exceed 20% per h.