Nonlinear anisotropic diffusion filtering of three-dimensional image data from two-photon microscopy
Nonlinear anisotropic diffusion filtering of three-dimensional image data from two-photon microscopy
复制标题
DOI：
10.1117/1.1806832
复制
发表时间：
2004-11-01
影响因子：
3.5
通讯作者：
Wittum, G
中科院分区：
文献类型：
--
作者：
Broser, PJ;Schulte, R;Wittum, G
双光子显微镜与新的荧光标记技术相结合，可以在完整的脑组织中成像三维神经元形态。原则上，现在可以从3-D荧光图像堆栈中自动重建神经元的树突分支模式。然而，在实践中，信噪比可能很低，特别是在组织中相对较深的树突或轴突成像的情况下。在这里，我们提出了一种非线性各向异性扩散滤波器，它在保持结构元素原始尺寸的同时提高了信噪比。其关键思想是利用原始数据中的结构信息--局部惯性矩--来局部控制扩散滤波的强度和方向。例如，柱状树枝晶只平行于其纵轴而不是垂直于其纵轴被有效地平滑。这一点在人工数据和来自大鼠大脑皮层锥体神经元的体内双光子显微镜数据中都得到了证明。在这两种情况下，噪声沿树枝平均，导致明显间隙的弥合，而树枝直径不受影响。该滤波器是平滑细胞过程的一个有价值的通用工具，非常适合为后续的图像分割和神经元重建准备数据。(C)2004年，光学仪器工程师学会。
Two-photon microscopy in combination with novel fluorescent labeling techniques enables imaging of three-dimensional neuronal morphologies in intact brain tissue. In principle it is now possible to automatically reconstruct the dendritic branching patterns of neurons from 3-D fluorescence image stacks. In practice however, the signal-to-noise ratio can be low, in particular in the case of thin dendrites or axons imaged relatively deep in the tissue. Here we present a nonlinear anisotropic diffusion filter that enhances the signal-to-noise ratio while preserving the original dimensions of the structural elements. The key idea is to use structural information in the raw data - the local moments of inertia - to locally control the strength and direction of diffusion filtering. A cylindrical dendrite, for example, is effectively smoothed only parallel to its longitudinal axis, not perpendicular to it. This is demonstrated for artificial data as well as for in vivo two-photon microscopic data from pyramidal neurons of rat neocortex. In both cases noise is averaged out along the dendrites, leading to bridging of apparent gaps, while dendritic diameters are not affected. The filter is a valuable general tool for smoothing cellular processes and is well suited for preparing data for subsequent image segmentation and neuron reconstruction. (C) 2004 Society of Photo-Optical Instrumentation Engineers.