Efficient vagina-to-lower respiratory tract immune trafficking in a murine model of influenza A virus infection
Efficient vagina-to-lower respiratory tract immune trafficking in a murine model of influenza A virus infection
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DOI：
10.1016/j.virol.2006.12.001
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发表时间：
2007-05-10
期刊：
影响因子：
3.7
通讯作者：
Castrucci, Maria Rita
中科院分区：
文献类型：
--
作者：
Garulli, Bruno;Meola, Monica;Castrucci, Maria Rita
有效的传染病疫苗接种策略考虑到记忆T细胞群体的诱导、长期维持和召回。为了了解黏膜间隔内的免疫串扰，我们比较了鼻内免疫和阴道免疫，结果表明，经阴道感染甲型流感病毒的BALB/c小鼠提供了保护粘膜免疫，以抵御呼吸道中同型和异型病毒的攻击。我们发现，在病毒攻击之前，经阴道免疫的小鼠肺内未检测到抗原特异性CD8+T细胞，血清抗体水平低于经鼻免疫的小鼠。然而，在肺部攻击后，NP147特异性CD8+T细胞在阴道免疫的小鼠中被招募和扩增，其程度与鼻内免疫的小鼠相同。因此，由流感病毒阴道免疫所引发的长期记忆免疫反应被有效地召回，并对呼吸道感染提供合理的保护。(C)2006 Elsevier Inc.保留所有权利。
Effective vaccination strategies for infectious diseases take into account the induction, long-term maintenance and recall of memory T-cell populations. To understand the immunological cross-talk within the mucosal compartments, we compared intranasal to vaginal immunization and demonstrated that vaginal infection of BALB/c mice with influenza A virus provides protective mucosal immunity against both homosubtypic and heterosubtypic virus challenge in the respiratory tract. We found that, prior to the viral challenge, in vaginally primed mice, antigen-specific CD8+ T cells were not detected in the lung airways and levels of serum antibodies were lower than those observed in intranasally immunized mice. However, following pulmonary challenge, NP147-specific CD8+ T cells were recruited and amplified in vaginally primed mice to the same extent as those in intranasally primed mice. Thus, the long-term memory immune response elicited by vaginal immunization with influenza virus is efficiently recalled and offers reasonable protection against infection in the respiratory tract. (c) 2006 Elsevier Inc. All rights reserved.