Standardization of Corneal Haze Measurement in Confocal Microscopy
Standardization of Corneal Haze Measurement in Confocal Microscopy
复制标题
DOI：
10.1167/iovs.10-5614
复制
发表时间：
2010-11-01
影响因子：
4.4
通讯作者：
Patel, Sanjay V.
中科院分区：
文献类型：
--
作者：
McLaren, Jay W.;Bourne, William M.;Patel, Sanjay V.
目的。在没有标准化的情况下，不能在纵向研究或跨实验室中比较角膜间质雾度。本研究设计了一种角膜共聚焦显微镜图像亮度的标准化方法。采用狭缝扫描共聚焦显微镜检查了18例未治疗患者的36个正常角膜和18例LASIK术后3年患者的35个角膜。通过Amco Clear （GSF Chemicals, Columbus， OH）溶液进行扫描，调整每帧的平均图像强度，以适应显微镜灵敏度的日常变化。调整后的图像强度以散点单位（SU）表示，表示与图像产生相同强度的Amco Clear浓度。采用广义估计方程模型比较LASIK术后角膜间质强度与未治疗角膜间质强度。在未经治疗的角膜中，前基质和中基质的图像亮度分别为1079 +/- 242和758 +/- 142 SU。LASIK术后3年，角膜瓣的图像强度为740 +/- 186 SU，比未治疗角膜的相应基质低约30% （P < 0.001）。LASIK术后角膜中基质亮度为715 +/- 117 SU，与未治疗角膜亮度无显著差异（P = 0.26）。临床共聚焦显微镜提供了角膜雾度的高分辨率测量，Amco Clear提供了标准化这些测量的手段。该方法可检测到LASIK术后3年角膜瓣内雾度的细微下降，也可用于板层角膜移植术后角膜瓣内雾度的变化。（Invest Ophthalmol Vis Sci. 2010;51:5610-5616） DOI:10.1167/iovs.10-5614
PURPOSE. Corneal stromal haze cannot be compared in longitudinal studies or across laboratories without standardization. In this study, a method was devised of standardizing image brightness in confocal microscopy of the cornea.METHODS. Thirty-six normal corneas of 18 untreated volunteers and 35 corneas of 18 patients 3 years after LASIK were examined by slit scanning confocal microscopy. The mean image intensity of each frame was adjusted for daily variations in sensitivity of the microscope by using scans through a solution of Amco Clear (GSF Chemicals, Columbus, OH). Adjusted image intensities were expressed in scatter units (SU), representing the concentration of Amco Clear that produced the same intensity as the image. The intensity from the stroma in corneas after LASIK was compared to that in untreated corneas by using generalized estimating equation models.RESULTS. In the untreated corneas, image brightness was 1079 +/- 242 and 758 +/- 142 SU in the anterior and mid stroma, respectively. Three years after LASIK, image intensity in the flap was 740 +/- 186 SU, approximately 30% lower than in corresponding stroma of the untreated corneas (P < 0.001). At mid stroma, brightness was 715 +/- 117 SU after LASIK, and was not significantly different from brightness in untreated corneas (P = 0.26).CONCLUSIONS. Clinical confocal microscopy provides a high-resolution measurement of corneal haze, and Amco Clear provides a means of standardizing these measurements. This method can detect subtle decreases in haze in the corneal flap 3 years after LASIK and could be used to examine changes in haze after lamellar keratoplasty. (Invest Ophthalmol Vis Sci. 2010;51:5610-5616) DOI:10.1167/iovs.10-5614