Isolation and identification of paralytic peptides from hemolymph of the lepidopteran insects Manduca sexta, Spodoptera exigua, and Heliothis virescens.
Isolation and identification of paralytic peptides from hemolymph of the lepidopteran insects Manduca sexta, Spodoptera exigua, and Heliothis virescens.
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鳞翅目昆虫天蛾、甜菜夜蛾和烟夜蛾血淋巴中麻痹肽的分离和鉴定。
DOI：
10.1016/s0021-9258(18)98775-2
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
G. B. Quistad
中科院分区：
文献类型：
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作者：
W. S. Skinner;P. A. Dennis;J. Li;R. Summerfelt;R. L. Carney;G. B. Quistad
从鳞翅目昆虫血淋巴中分离鉴定了7种麻痹肽。当注射到天蛾和其他一些鳞翅目幼虫中时，所有这些肽都会引起快速的僵硬麻痹。每个肽含有23个氨基酸残基，包括2个半胱氨酸，羧基末端是酸性的。二硫化物或还原形式的合成肽以及羧基末端的酸或酰胺具有同样的麻痹性。最有效的麻痹肽Mas PP I具有以下序列：H-Glu-Asn-Phe-Ala-Gly-Gly-Cys-Ala-Thr-Gly-Tyr-Leu- Arg-Thr-Ala-Asp-Gly-Arg-Cys-Lys-Pro-Thr-Phe-OH。从M. sexta血淋巴的显著之处在于它们是自身麻痹的（即，当注射到相同的幼虫中时，收集的血淋巴中的因子是麻痹的）。
Seven paralytic peptides were isolated and identified from lepidopteran hemolymph. All of these peptides cause rapid, rigid paralysis when injected into Manduca sexta and some other lepidopteran larvae. Each peptide contains 23 amino acid residues including 2 cysteines and the carboxyl termini are acidic. Synthetic peptides in the disulfide or reduced forms, and as carboxyl-terminal acids or amides were equally paralytic. The most potent paralytic peptide, Mas PP I, has the following sequence: H-Glu-Asn-Phe-Ala-Gly-Gly-Cys-Ala-Thr-Gly-Tyr-Leu- Arg-Thr-Ala-Asp-Gly-Arg-Cys-Lys-Pro-Thr-Phe-OH. The two peptides from M. sexta hemolymph are remarkable in that they are autoparalytic (i.e. factors in collected hemolymph that are paralytic when injected into the same larvae).