A New Method of Sample Preparation for Kenaf Bast Fiber Length Analysis with Automated Fiber Length Analyzer
A New Method of Sample Preparation for Kenaf Bast Fiber Length Analysis with Automated Fiber Length Analyzer
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自动化纤维长度分析仪分析红麻麻纤维长度样品制备的新方法
DOI：
10.1515/hf.2000.036
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
K. Sameshima
中科院分区：
文献类型：
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作者：
Zhou Cheng;S. Fujiwara;Y. Ohtani;K. Sameshima
红麻韧皮部纤维束呈放射状分层排列，并伴有筛分子和韧皮薄壁细胞。红麻韧皮横截面面积中纤维束约占20%.对红麻韧皮纤维长度自动分析仪的4种制样方法进行了比较，确定了一种新的3步制样方法。纤维束是通过酸化的碳酸钠处理和附加的草酸铵处理获得的。在1%氢氧化钠处理后，纤维束完全分离成单纤维而没有损伤。该方法的纤维长度值比其他三种样品制备方法和光学显微镜手工测量方法更准确、可靠。
Summary Kenaf bast fiber bundles alternate radially in layers accompanied by sieve elements and phloem parenchyma cells. Fiber bundles are about 20 % in the area of a cross section of kenaf bast. Four sample preparation methods for kenaf bast fiber length analysis with an automated fiber length analyzer were compared and a new sample preparation method including three steps was proven to be accurate. Fiber bundles were obtained by acidified sodium chlorite treatment and an additional ammonium oxalate treatment. Following a 1% sodium hydroxide treatment, fiber bundles were completely separated into single fibers with no damage. Fiber length values of this method were more accurate and reliable than other three sample preparation methods and manual measurement with optical microscope.