PRIMARY OLFACTORY FIBERS PROJECT TO THE VENTRAL TELENCEPHALON AND PREOPTIC REGION IN TROUT (SALMO-TRUTTA) - A DEVELOPMENTAL IMMUNOCYTOCHEMICAL STUDY
PRIMARY OLFACTORY FIBERS PROJECT TO THE VENTRAL TELENCEPHALON AND PREOPTIC REGION IN TROUT (SALMO-TRUTTA) - A DEVELOPMENTAL IMMUNOCYTOCHEMICAL STUDY
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DOI：
10.1002/cne.903420112
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发表时间：
1994-04-01
影响因子：
2.5
通讯作者：
ANADON, R
中科院分区：
文献类型：
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作者：
BECERRA, M;MANSO, MJ;ANADON, R
我们研究了硬骨鱼，褐鳟鱼的初级嗅觉系统的发展，目的是澄清尾侧投射是否属于嗅觉或终端神经系统，识别接受此投射的脑区，并调查其可能的功能意义。作为嗅觉标记物（OMs），我们使用了两种多克隆抗体（物质P和α-黑素细胞刺激激素），发现标记的嗅觉投射强烈的预吸附后的抗体与相应的抗原（OMs），并作为一个终端的神经标记物，我们使用了抗血清FMRF-酰胺肽。OM标记见于嗅神经元胞体和轴突。在成年人中，嗅觉神经元不仅投射到嗅球中的嗅球，而且如先前所报道的那样，通过内侧嗅束投射到前脑中更多的尾侧目标。我们的研究结果表明，这些目标包括腹侧和连合区端脑，脑室周围视前区，和终板血管器。肾小球在孵化前没有观察到，球外嗅觉投射出现在发育后期。广泛的脑室周围视前嗅觉神经丛和嗅神经支配的终板血管器没有出现，直到成年。终神经节的细胞，形成神经节组沿着嗅觉神经，没有染色与这些嗅觉标记物在任何发展阶段的研究，也不是内侧嗅道FMRF-酰胺肽免疫反应。因此，我们的研究结果证实了初级嗅觉投射到球外目标鳟鱼的存在。在这项研究中确定的目标区域有牵连的性行为：我们讨论了相关的可能性，在硬骨鱼，这些球外嗅觉投射（而不是终端神经的预测，因为被广泛认为）的主要介质的神经内分泌反应信息素。(C)1994 Wiley-Liss，Inc.
We studied the development of the primary olfactory system of a teleost, the brown trout, with the aims of clarifying whether the caudal projection pertains to the olfactory or to the terminal nerve system, of identifying the brain regions receiving this projection, and of investigating its possible functional significance. As olfactory markers (OMs) we used two polyclonal antibodies (to substance P and to a-melanocyte-stimulating hormone) that were found to label the olfactory projection strongly after preadsortion of the antibody with the corresponding antigen (OMs), and as a terminal nerve marker we used an antiserum to FMRF-amide peptide. OM labelling was observed in both perikarya and axons of olfactory neurons. In adults, olfactory neurons projected not only to olfactory glomeruli in the olfactory bulb but also, as has been reported previously, to more caudal targets in the forebrain through the medial olfactory tract. Our results show that these targets include the ventral and commissural nuclei of the area ventralis telencephali, the periventricular preoptic region, and the organum vasculosum laminae terminalis. Glomeruli were not observed before hatching, and the extrabulbar olfactory projections appear late in development. Extensive periventricular preoptic olfactory plexuses and olfactory innervation of the organum vasculosum laminae terminalis did not appear until adulthood. The cells of the ganglion nervus terminalis, which form ganglionic groups along the olfactory nerves, were not stained with these olfactory markers at any developmental stage studied, nor was the medial olfactory tract FMRF-amide peptide immunoreactive. Our results thus confirm the existence of primary olfactory projections to extrabulbar targets in trout. The target regions identified in this study are implicated in sexual behaviour: We discuss the related possibility that, in teleosts, these extrabulbar olfactory projections (rather than projections of the terminal nerve, as is widely held) are the primary mediators of neuroendocrine response to pheromones. (C) 1994 Wiley-Liss, Inc.