SCHWANN-CELL GALACTOCEREBROSIDE INDUCED BY DERIVATIVES OF ADENOSINE-3',5'-MONOPHOSPHATE
SCHWANN-CELL GALACTOCEREBROSIDE INDUCED BY DERIVATIVES OF ADENOSINE-3',5'-MONOPHOSPHATE
复制标题
DOI：
10.1126/science.6322307
复制
发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
PLEASURE, D
中科院分区：
文献类型：
--
作者：
SOBUE, G;PLEASURE, D
用间接免疫荧光法显示apprx表面存在半乳糖脑苷脂(GalC)，这是一种发现于髓鞘中的脂质。1/2雪旺细胞，分离自新生大鼠坐骨神经，无神经元培养1d。在体外培养的第4天，雪旺细胞表面的GalC已全部消失。10-3M 8-溴cAMP或二丁酰cAMP作用3d后，雪旺细胞表面又出现GalC，2d后48%的雪旺细胞表面出现GalC。经8-溴cAMP和二丁酰cAMP处理的培养物以15倍的速率将~3H-D-半乳糖的氚掺入GalC。
Indirect immunofluroescence was used to show the presence of galactocerebroside (galC), a lipid found in myelin, on the surface of .apprx. 1/2 of the Schwann cells, isolated from neonatal rat sciatic nerves and cultured for 1 day without neurons. By day 4 in vitro, the Schwann cells had all lost their surface galC. Three days after beginning treatment with 10-3 M 8-bromo cAMP or dibutyryl cAMP, galC reappeared on the Schwann cells, and 2 days later 48% of the cultured Schwann cells showed surface galC. Tritium from 3H D-galactose was incorporated into galC by the 8-bromo cAMP- and dibutyryl cAMP-treated cultures at a rate 15 times the control rate.