Ultrastructural localization of the CD68 macrophage-associated antigen in human blood neutrophils and monocytes.
Ultrastructural localization of the CD68 macrophage-associated antigen in human blood neutrophils and monocytes.
复制标题
CD68 巨噬细胞相关抗原在人血液中性粒细胞和单核细胞中的超微结构定位。
DOI：
--
复制
发表时间：
1991
影响因子：
6
通讯作者：
E. Cramer
中科院分区：
文献类型：
--
作者：
N. Saito;K. Pulford;J. Breton‐Gorius;J. Massé;D. Mason;E. Cramer
CD 68抗原，110 kd的细胞内糖蛋白与髓系细胞和单核细胞/巨噬细胞，超微结构的定位进行了研究，在人中性粒细胞的植入后免疫金染色，使用单克隆抗体KP 1。在中性粒细胞的初级颗粒中发现抗原，尽管并非所有初级颗粒都被标记。它不存在于质膜上。在单核细胞中，它也被检测到细胞质颗粒，共定位与溶菌酶和髓过氧化物酶。这一观察结果证实并完善了免疫荧光和其他光学显微镜方法获得的结果。此外，该研究表明，由抗体KP 1识别的CD 68表位能够抵抗固定和嵌入，因此强调了使用KP 1作为该巨噬细胞相关分子的标记物的价值。
The ultrastructural localization of the CD68 antigen, a 110-kd intracellular glycoprotein associated with myeloid cells and with monocytes/macrophages, was investigated in human neutrophil granulocytes by postembedding immunogold staining, using monoclonal antibody KP1. The antigen was found in the primary granules of neutrophils, although not all primary granules were labeled. It was absent from the plasma membrane. In monocytes, it was also detected within cytoplasmic granules, colocalized with lysozyme and myeloperoxidase. This observation confirms and completes results obtained by immunofluorescence and other light-microscopic methods. Moreover this study shows that the CD68 epitope recognized by antibody KP1 is able to resist fixation and embedment and therefore emphasizes the value of using KP1 as a marker for this macrophage-associated molecule.
富含防御素的人类中性粒细胞致密颗粒。
影响因子：
20.3
作者：
Rice,WG;Ganz,T;KinkadeJr,JM;Selsted,ME;Lehrer,RI;Parmley,RT
通讯作者：
Parmley,RT
人中性粒细胞中含有过氧化物酶和弹性蛋白酶的颗粒的超微结构定量。
DOI：
--
发表时间：
1988
期刊：
The American journal of pathology
影响因子：
--
作者：
Damiano,VV;Kucich,U;Murer,E;Laudenslager,N;Weinbaum,G
通讯作者：
Weinbaum,G