Effects of Different Dietary Vitamin E Levels on Growth Performance, Non-specific Immune Responses, and Disease Resistance against Vibrio anguillarum in Parrot Fish (Oplegnathus fasciatus)
Effects of Different Dietary Vitamin E Levels on Growth Performance, Non-specific Immune Responses, and Disease Resistance against Vibrio anguillarum in Parrot Fish (Oplegnathus fasciatus)
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DOI：
10.5713/ajas.2010.90494
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发表时间：
2010-07-01
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影响因子：
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通讯作者：
Lee, Kyeong-Jun
中科院分区：
文献类型：
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作者：
Galaz, German Bueno;Kim, Sung-Sam;Lee, Kyeong-Jun
本文首次报道了维生素E在鹦鹉鱼中的营养生理和非特异性免疫反应。本研究旨在根据鹦鹉鱼的生长性能、非特异性免疫反应和抗角弧菌的攻毒试验，探讨维生素E在鹦鹉鱼饲料中的必要性和需要量。在0、25、50、75、100和500 mg/kg饲料下（指定为E0、E25、E50、E75、E100和E500），在饲养试验结束时，E0、E25、1350、E75、E100和E500的维生素E的分析饮食浓度分别为0、38、53、87、119和538 mg/kg饮食。喂食E25的鱼的生长性能和饲料利用率显著高于喂食其它饮食的鱼。肝脏α-生育酚浓度以剂量依赖性方式随着饮食α-TA的增加而显著增加。在测定的免疫应答中，在饲喂基础饲料12周的鱼中未观察到维生素E缺乏和死亡的明显临床体征。在鳗弧菌、E75、E100攻毒试验中，随着饲料中α-TA水平的增加，吞噬细胞（NBT法）和髓过氧化物酶活性显著增加。E500组成活率高于E0、E25和E50组。本研究结果表明，鹦鹉鱼需要外源维生素E，其最适添加量为38 mg/kg饲料，以维持正常生长和生理。为了增强鹦鹉鱼幼鱼对鳗弧菌的非特异性免疫应答，提高鹦鹉鱼幼鱼对鳗弧菌的抵抗力，需要添加500 mg/kg以上的饲料。
We report nutritional physiology and non-specific immune responses of vitamin E in parrot fish for the first time This study aimed to investigate the essentiality and requirements in diets based on growth performances, non-specific immune responses and a challenge test against Vibrio angullarum Six casein-gelatin based semi-purified diets were formulated to contain six graded levels of DL-alpha-tocopheryl acetate (alpha-TA) at 0, 25, 50, 75, 100 and 500 mg/kg diet (designated as E0, E25, E50, E75, E100 and E500. respectively) and fed to triplicate groups of Juvenile parrot fish for 12 weeks The analyzed dietary concentrations of vitamin E were 0, 38, 53, 87, 119 and 538 mg/kg diet for E0, E25, 1350, E75, E100 and E500, respectively At the end of the feeding trial. growth performance and feed utilization of fish fed the E25 were significantly higher compared to that of fish fed the other diets Liver alpha-tocopherol concentration was significantly increased with an increase in dietary alpha-TA in a dose dependent manner. No apparent clinical signs of vitamin E deficiency and mortality were observed in fish fed the basal diet for 12 weeks Among the immune responses assayed. phagocytic (NBT assay) and myeloperoxidase activities were significantly increased with increment of dietary alpha-TA levels During the challenge test with V anguillarum, E75, E100. and E500 diets resulted in higher survival than E0, E25 and E50 diets. The findings of this study suggest that parrot fish require exogenous vitamin E and the optimum dietary level could be approximately 38 mg alpha-TA/kg diet for normal growth and physiology Dietary alpha-TA concentration over 500 mg/kg could be required to enhance the non-specific immune responses and improve the resistance of juvenile parrot fish against V anguillarum.