Immunomodulatory action of levofloxacin on cytokine production by human peripheral blood mononuclear cells.
Immunomodulatory action of levofloxacin on cytokine production by human peripheral blood mononuclear cells.
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左氧氟沙星对人外周血单核细胞产生细胞因子的免疫调节作用。
作者：
Yoshimura;Tomoaki;Kurita;Chikako;Usami;Eiseki;Nakao;Toshiya;Watanabe;Shino;Kobayashi;Joji;Yamazaki;Futoshi;Nagai;Hiroichi
左氧氟沙星（LVFX）是氧氟沙星的细菌活性异构体，是一种氟喹诺酮。LVFX抑制植物血凝素（PHA）刺激的外周血单个核细胞（PBMC）的增殖活性。LVFX以剂量依赖性方式增加PHA刺激的PBMC产生的白细胞介素-2（IL-2），超过10微克/ml的LVFX引起显著增加。高浓度LVFX抑制PHA刺激PBMC产生的粒细胞-巨噬细胞集落刺激因子和可溶性IL-2受体。LVFX以浓度依赖性方式抑制脂多糖刺激的PBMC产生白细胞介素-1 β，仅在最高浓度下抑制肿瘤坏死因子-α的产生。与此相反，白细胞介素-8的生产几乎没有影响LVFX。这些结果表明，LVFX对PBMC产生的细胞因子具有免疫调节作用，与其抗微生物活性无关。
Levofloxacin (LVFX), the bacteriologically active isomer of ofloxacin, is a fluorinated quinolone. LVFX suppressed the proliferative activity of peripheral blood mononuclear cells (PBMC) stimulated with phytohemagglutinin (PHA). LVFX increased interleukin-2 (IL-2) production by PBMC stimulated with PHA in a dose-dependent manner, with more than 10 micrograms/ml of LVFX causing a significant increase. The granulocyte-macrophage colony-stimulating factor and soluble IL-2 receptor production by PHA-stimulated PBMC was suppressed at high concentrations of LVFX. Interleukin-1 beta production by lipopolysaccharide-stimulated PBMC was suppressed in a concentration-dependent manner by LVFX, and tumor necrosis factor-alpha production was suppressed at only the highest concentration. In contrast, interleukin-8 production was little affected by LVFX. These results show that LVFX has an immunomodulatory action on cytokines production by PBMC independent of its antimicrobial activity.