Absence of force suppression in rabbit bladder correlates with low expression of heat shock protein 20.
Absence of force suppression in rabbit bladder correlates with low expression of heat shock protein 20.
复制标题
兔膀胱中没有力抑制与热激蛋白20的低表达相关。
DOI：
10.1186/1472-6793-5-16
复制
发表时间：
2005-11-02
期刊：
影响因子：
--
通讯作者：
Rembold CM
中科院分区：
文献类型：
--
作者：
Batts TW;Walker JS;Murphy RA;Rembold CM
Nitroglycerin can induce relaxation of swine carotid artery without sustained reductions in [Ca2+]i or myosin regulatory light chain (MRLC) phosphorylation. This has been termed force suppression and been found to correlate with ser16-phosphorylation of heat shock protein 20 (HSP20). We tested for the existence of this mechanism in a smooth muscle that is not responsive to nitric oxide. Isometrically mounted mucosa free rabbit bladder strips were contracted with carbachol and relaxed with 8-Br-cGMP, forskolin, or isoprenaline. Contraction was associated with a highly cooperative relation between MRLC phosphorylation and force such that very small increases in MRLC phosphorylation induced large increases in force. Relaxation induced by 8-Br-cGMP, forskolin, or isoprenaline did not shift the MRLC phosphorylation-force relation from that observed with carbachol alone, i.e. there was no force suppression. HSP20 content was negligible (approximately two hundred-fold less than swine carotid). The lack of force suppression in the absence of HSP20 is consistent with the hypothesized role for HSP20 in the force suppression observed in tonic smooth muscles.
