Hormonal Control of Morphogenesis in Leaf Explants of Perilla frutescens Britton var. crispa Decaisne f. viridi-crispa Makino
Hormonal Control of Morphogenesis in Leaf Explants of Perilla frutescens Britton var. crispa Decaisne f. viridi-crispa Makino
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紫苏叶外植体形态发生的激素控制。
DOI：
10.1093/oxfordjournals.aob.a085827
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
H. Harada
中科院分区：
文献类型：
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作者：
S. Tanimoto;H. Harada
以紫苏子（Perilla frutescensvar.crispaf. viridi-crispa）叶圆片为材料，研究了影响紫苏子芽和根形成、愈伤组织诱导、体细胞胚胎发生和花芽形成的因素。培养基中添加萘乙酸（NAA）可使愈伤组织致密，而添加2，4-二氯苯氧乙酸（2，4-D）可促进外植体表面形成柔软易碎的愈伤组织。苄基腺嘌呤，当与生长素，抑制愈伤组织和根的形成。外植体先在含有2，4-D和有机元素的营养培养基上生长，然后转移到不含2，4-D的培养基上，即可发生体细胞胚。用细胞分裂素、N-苯基-N '-（4-吡啶基）脲及其衍生物处理诱导芽形成。低浓度的NAA和萘氧乙酸促进芽的发育。偶尔的花芽形成观察到的原始叶位置从叶盘被切除的母株。花芽形成能力沿着茎呈梯度变化。
Leaf discs ofPerilla frutescensvar.crispaf.viridi-crispawere cultured on a defined medium to investigate factors influencing bud and root formation, callus induction, somatic embryogenesis, and floral bud formation. Addition of naphthalene-acetic acid (NAA) to the culture medium caused compact callus whereas 2,4-dichlorophenoxy acetic acid (2,4-D) promoted soft and friable callus formation on the surface of the explants. Benzyladenine, when applied with auxin, suppressed callus and root formation. Somatic embryogenesis occurred, when the explants were first grown on nutrient medium containing 2,4-D and organic elements, and then transferred to the 2,4-D free medium. Treatments with cytokinins,N-phenyl-N'-(4-pyridyl) urea and its derivatives induced bud formation. A low concentration of NAA and naphthoxy-acetic acid promoted bud development. Occasional floral bud formation was observed depending on the original leaf positions on mother plants from which the leaf discs were excised. A gradient of floral bud forming capacity along the stem was noted.