Molecular determinants controlling NMDA receptor synaptic incorporation.
Molecular determinants controlling NMDA receptor synaptic incorporation.
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DOI：
10.1523/jneurosci.5553-10.2011
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发表时间：
2011-04-27
期刊：
影响因子：
--
通讯作者：
Barria A
中科院分区：
文献类型：
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作者：
Storey GP;Opitz-Araya X;Barria A
NMDARs的突触结合受GluN2亚基调控，其不同的规则控制GluN2A-和glun2b -含受体；虽然含有GluN2B的受体组成性地结合到突触中，但GluN2A的结合是活性依赖的。我们在大鼠海马切片中表达电生理标记的NMDARs，以确定控制NMDARs突触结合模式的分子决定因素。通过表达嵌合GluN2亚基，我们确定了GluN2B中存在一个假定的n -糖基化位点，而不是GluN2A，这是驱动NMDARs以不依赖于活性的方式进入突触的必要和充分条件。这提示了一种新的机制来调节活动驱动的变化和NMDARs到突触的运输。
Synaptic incorporation of NMDARs is regulated by GluN2 subunits with different rules controlling GluN2A- and GluN2B-containing receptors; while GluN2B containing receptors are constitutively incorporated into synapses, GluN2A incorporation is activity-dependent. We expressed electrophysiologically tagged NMDARs in rat hippocampal slices in order to identify the molecular determinants controlling the mode of synaptic incorporation of NMDARs. Expressing chimeric GluN2 subunits, we identified a putative N-glycosylation site present in GluN2B, but not on GluN2A, as necessary and sufficient to drive NMDARs into synapses in an activity-independent manner. This suggests a novel mechanism for regulating activity driven changes and trafficking of NMDARs to the synapse.