Traction in smooth muscle cells varies with cell spreading
Traction in smooth muscle cells varies with cell spreading
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DOI：
10.1016/j.jbiomech.2004.06.027
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发表时间：
2005-07-01
影响因子：
2.4
通讯作者：
Wang, N
中科院分区：
文献类型：
--
作者：
Tolic-Norrelykke, IM;Wang, N
细胞形状的变化调节细胞生长、分化和凋亡。有人认为，细胞形状对细胞功能的调节是细胞骨架张力和细胞变形的结果。在这里，我们探讨了细胞产生的机械力与细胞形态之间的关联。我们假设细胞收缩力与细胞铺展程度相关，特别是与细胞长度相关。我们测量了铺在聚丙烯酰胺凝胶上的单个人气道平滑肌细胞的牵引场，其中嵌入荧光微珠作为凝胶变形的标记。细胞在细胞-基质界面处施加的牵引力由测量的凝胶变形确定。在用收缩激动剂组胺或舒张激动剂异丙肾上腺素治疗之前和之后测量牵引力。组胺引起的牵引力的相对增加与基线牵引力呈负相关。相反，异丙肾上腺素引起的牵引力相对下降与基线牵引力无关，但与细胞形状相关：细长细胞的牵引力比圆形细胞下降更多。在本研究中，最大细胞宽度、平均细胞宽度和细胞投影面积是与基线和组胺诱导的牵引力最紧密相关的参数。宽且分布良好的细胞比细小的细胞产生更大的牵引力。这些结果表明细胞收缩性是由细胞扩散控制的。 (c) 2004 Elsevier Ltd. 保留所有权利。
Changes in cell shape regulate cell growth, differentiation, and apoptosis. It has been suggested that the regulation of cell function by the cell shape is a result of the tension in the cytoskeleton and the distortion of the cell. Here we explore the association between cell-generated mechanical forces and the cell morphology. We hypothesized that the cell contractile force is associated with the degree of cell spreading, in particular with the cell length. We measured traction fields of single human airway smooth muscle cells plated on a polyacrylamide gel, in which fluorescent microbeads were embedded to serve as markers of gel deformation. The traction exerted by the cells at the cell-substrate interface was determined from the measured deformation of the gel. The traction was measured before and after treatment with the contractile agonist histamine, or the relaxing agonist isoproterenol. The relative increase in traction induced by histamine was negatively correlated with the baseline traction. On the contrary, the relative decrease in traction due to isoproterenol was independent of the baseline traction, but it was associated with cell shape: traction decreased more in elongated than in round cells. Maximum cell width, mean cell width, and projected area of the cell were the parameters most tightly coupled to both baseline and histamine-induced traction in this study. Wide and well-spread cells exerted larger traction than slim cells. These results suggest that cell contractility is controlled by cell spreading. (c) 2004 Elsevier Ltd. All rights reserved.