Evaluation of resting traps to examine the behaviour and ecology of mosquito vectors in an area of rapidly changing land use in Sabah, Malaysian Borneo
Evaluation of resting traps to examine the behaviour and ecology of mosquito vectors in an area of rapidly changing land use in Sabah, Malaysian Borneo
复制标题
DOI：
10.1186/s13071-018-2926-1
复制
发表时间：
2018-06-14
影响因子：
3.2
通讯作者：
Ferguson, Heather M.
中科院分区：
文献类型：
--
作者：
Brown, Rebecca;Hing, Chua Tock;Ferguson, Heather M.
背景：热带地区普遍发生的森林砍伐被认为会影响媒介传播疾病(VBD)的传播。预测环境变化将如何影响VBD的传播取决于对潜在媒介物种在家庭环境之外的生态和行为的了解。然而，几乎没有可靠的抽样工具来衡量蚊媒的栖息地偏好和宿主选择；几乎没有适合对最近吸血、休息的蚊子进行抽样的工具。这项研究评估了两种捕蚊器的使用：休息桶(RB)和粘性休息桶(SRB)陷阱相对于CDC背包吸引器(CDC)，用于采样在代表森林砍伐梯度的一系列栖息地休息的蚊子。在马来西亚婆罗洲库达特区的两个村庄周围选择了8个栖息地进行采样，以反映蚊子在人类居住地及其周围以及附近有水库宿主的森林栖息地的可用栖息地的范围：次生林(边缘、内部和树冠)；人工林(棕榈树和橡胶)；以及人居环境(房屋内、房屋下和房屋周围)。结果：在31天的时间里，5748个离散的收集到2243只蚊子。共采集了9个蚊属，其中伊蚊和库蚊在所有生境中均有存在，且数量最多。RB和CDC背包抽吸对库蚊的抽吸效率最高，而CDC背包抽吸和SRB对伊蚊的抽吸效率最高。在物种水平上鉴定的伊蚊多为伊蚊。白纹伊蚊(91%)，其丰度在林缘生境中最高。相比之下，库蚊在房屋下的数量最多。以血为食的蚊虫以居民点居多(76%)，人和鸡是唯一的血源。结论：RB和SRB诱捕器可以在所有采样的栖息地采集栖息蚊子。然而，抽样效率普遍较低(每个捕蚊器每天约0.1个)，因此必须大量部署捕蚊器以检测蚊子。没有一种诱捕器对人畜共患疟疾病媒的抽样有效；然而，SRB收集了相对较高数量的登革热病媒Ae。白纹伊蚊。森林边缘栖息地的蚊子丰度较高，这表明这些陷阱在调查高山登革热传播方面具有潜在的价值。这项研究证明了使用简单的捕蚊器来表征蚊子媒介在户外的休息行为的优点。
Background: Widespread deforestation occurring in the tropics is hypothesized to impact the transmission of vector-borne diseases (VBD). Predicting how environmental changes will impact VBD transmission is dependent on understanding the ecology and behaviour of potential vector species outside of domestic settings. However there are few reliable sampling tools for measuring the habitat preference and host choice of mosquito vectors; with almost none suitable for sampling recently blood-fed, resting mosquitoes. This study evaluated the use of two mosquito traps: the resting bucket (RB) and sticky resting bucket (SRB) traps relative to CDC backpack aspiration (CDC) for sampling mosquitoes resting in a range of habitats representing a gradient of deforestation. Eight habitats were selected for sampling around two villages in Kudat District, Malaysian Borneo, to reflect the range of habitats available to mosquitoes in and around human dwellings, and nearby forest habitats where reservoir hosts are present: secondary forest (edge, interior and canopy); plantations (palm and rubber); and human settlements (inside, under and around houses).Results: Over 31 days, 2243 mosquitoes were collected in 5748 discrete collections. Nine mosquito genera were sampled with Aedes and Culex species being present in all habitats and most abundant. RB and CDC backpack aspiration were most efficient for sampling Culex whereas CDC backpack aspiration and SRB were most efficient for Aedes. Most Aedes identified to species level were Ae. albopictus (91%), with their abundance being highest in forest edge habitats. In contrast, Culex were most abundant under houses. Most blood-fed mosquitoes (76%) were found in human settlements; with humans and chickens being the only blood source.Conclusions: RB and SRB traps proved capable of sampling mosquitoes resting in all sampled habitats. However, sampling efficiency was generally low (c.0.1 per trap per day), necessitating traps to be deployed in high numbers for mosquito detection. None of the traps were effective for sampling zoonotic malaria vectors; however, SRB collected relatively higher numbers of the dengue vector Ae. albopictus. The higher abundance of mosquitoes in forest edge habitats indicates the potential value of these traps for investigating sylvatic dengue transmission. This study has demonstrated the merits in application of simple resting traps for characterising mosquito vector resting behaviour outside of the home.