Highly enantioselective reduction of 2-hydroxy-1-phenylethanone to enantiopure (R)-phenyl-1,2-ethanediol using Saccharomyces cerevisiae of remarkable reaction stability
Highly enantioselective reduction of 2-hydroxy-1-phenylethanone to enantiopure (R)-phenyl-1,2-ethanediol using Saccharomyces cerevisiae of remarkable reaction stability
复制标题
使用具有出色反应稳定性的酿酒酵母将 2-羟基-1-苯基乙酮高度对映选择性还原为对映体纯 (R)-苯基-1,2-乙二醇
DOI：
10.1016/j.biortech.2010.06.044
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发表时间：
2010-11-01
影响因子：
11.4
通讯作者：
Nie, Yao
中科院分区：
文献类型：
--
作者：
Hu, Qingsen;Xu, Yan;Nie, Yao
通过目标反应导向筛选，成功获得了一株能将2-羟基-1-苯基乙酮（HPE）还原为（R）-苯基-1，2-乙酮（（R）-PED）的酿酒酵母JUC 15。>99.9%）。当游离细胞在2 g L-1底物浓度下重复使用40个循环时，（R）-PED的产率和光学纯度没有显著降低。该菌株使用廉价的蔗糖进行辅因子再生，并在相当宽的pH范围（4-9）内工作。最适底物浓度为8 g L-1，高于目前报道的其它生物催化剂。有趣的是，当反应体系中HPE浓度达到20 g L-1时，其中43.2%的底物以悬浮固体形式存在，反应仍然以71%的产率得到对映体纯的（R）-PED。最后但并非最不重要的是，产品e. e。在所有检测条件下保持在99.9%以上。这些结果表明该菌株具有工业生产光学活性（R）-PED的潜力。(C)2010爱思唯尔有限公司保留所有权利。
Saccharomyces cerevisiae JUC15 was successfully obtained by target reaction-oriented screening, which reduced 2-hydroxy-1-phenylethanone (HPE) to (R)-phenyl-1,2-ethanediol ((R)-PED) of excellent enantiomeric excess (e.e. >99.9%). There was no significant decrease in the yield and optical purity of (R)-PED when the free cells were reused for 40 repeated cycles at 2 g L-1 substrate concentration. The strain used cheap sucrose for cofactor regeneration and worked over a considerably wider range of pH (4-9). The optimum substrate concentration was 8 g L-1, which was higher than any other biocatalysts reported so far. Interesting, when HPE concentration reached 20 g L-1 in reaction system, where 43.2% of the substrate was present in suspended solid form, the reaction still gave enantiopure (R)-PED in 71% yield. Last but not least, the product e.e. kept above 99.9% in all examined conditions. These results suggest the potential of this strain for the industrial production of optically active (R)-PED. (C) 2010 Elsevier Ltd. All rights reserved.