High expression of heat shock protein 60 in follicular cells of Hashimoto's thyroiditis
High expression of heat shock protein 60 in follicular cells of Hashimoto's thyroiditis
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DOI：
10.3109/08916939608994721
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发表时间：
1996-01-01
期刊：
影响因子：
3.5
通讯作者：
Ohtaki, S
中科院分区：
文献类型：
--
作者：
Kotani, T;Aratake, Y;Ohtaki, S
采用免疫组织化学方法研究了桥本甲状腺炎和格雷夫斯病甲状腺细胞中热休克蛋白 60 的表达。桥本甲状腺炎的甲状腺细胞不仅在高的嗜酸性细胞中高表达热休克蛋白60，而且在低的和扁平的细胞中也有高表达，尽管前者的染色强度为中至强，后者的染色强度为弱至中度，淋巴细胞滤泡周围的滤泡细胞染色较强烈，而淋巴细胞滤泡以外的细胞染色为弱至中度。在格雷夫斯病中，仅周围的滤泡细胞染色。淋巴样细胞。滤泡以不同强度染色，由于所有阳性染色的模式都是颗粒状的，因此认为热休克蛋白 60 在位于线粒体的甲状腺细胞中过表达。为了研究自身热休克蛋白 60 在桥本甲状腺炎的甲状腺细胞中过表达的免疫学作用，研究了组织中的 γ δ TCR(+) T 细胞和抗自身热休克蛋白 60 抗体的 IgG 类，γ δ在分散于滤泡间结缔组织的淋巴样细胞中检测到TCR(+) T细胞，桥本甲状腺炎、格雷夫斯病和正常受试者之间抗体水平没有差异，桥本甲状腺炎甲状腺细胞中自身热休克蛋白60过表达可能起到缓解病情的作用，但不影响抗自身热休克蛋白60抗体水平。
Heat shock protein 60 expression in the thyrocytes of Hashimoto's thyroiditis and Graves' disease was studied immunohistochemically. Thyrocytes of Hashimoto's thyroiditis showed high expression of heat shock protein 60 not only in tall eosinophilic-cells but also in low and flattened cells, although the former was stained with moderate to strong staining intensity and the latter weakly to moderately, Follicular cells around lymphoid cell follicles were stained more intensely, whereas cells apart from lymphoid cell follicles were stained weakly to moderately, In Graves' disease, only follicular cells around lymphoid cell. follicles were stained with varying intensities, Since the pattern in all positive staining was granular, it was thought that heat shock protein 60 overexpressed in thyrocytes located on mitochondria.To investigate the immunological role of overexpression of self heat shock protein 60 in the thyrocytes of Hashimoto's thyroiditis, gamma delta TCR(+) T-cells in the tissue and the IgG class of anti-self heat shock protein 60 antibodies were studied, gamma delta TCR(+) T-cells were detected among lymphoid cells scattered in interfollicular connective tissue, No difference in antibody level was seen among subjects with Hashimoto's thyroiditis, Graves' disease and normal subjects, Self heat shock protein 60 overexpression in the thyrocytes of Hashimoto's thyroiditis may play a disease-modifying role, although it does not influence the anti-self heat shock protein 60 antibody level.