Up-regulation of gastric cancer cell invasion by Twist is accompanied by N-cadherin and fibronectin expression
Up-regulation of gastric cancer cell invasion by Twist is accompanied by N-cadherin and fibronectin expression
复制标题
Twist 上调胃癌细胞侵袭伴随着 N-钙粘蛋白和纤连蛋白表达
DOI：
10.1016/j.bbrc.2007.05.023
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发表时间：
2007-07-06
影响因子：
3.1
通讯作者：
Wu, Xiaoting
中科院分区：
文献类型：
--
作者：
Yang, Zhou;Zhang, Xiaohong;Wu, Xiaoting
Twist是一种新发现的EMT诱导因子，在N-cadherin高表达的弥漫型胃癌中表达上调。我们在这里显示MKN 45，一种来源于未分化癌细胞的细胞系，表达高水平的Twist。在MKN 45细胞中使用反义Twist载体下调Twist，抑制细胞迁移和侵袭，伴随与MET相关的形态学变化。Twist的抑制也降低了MKN 45中N-钙粘蛋白和纤连蛋白的表达，但不降低E-钙粘蛋白的表达。与此相反，在MKN 28，一种来源于中度分化癌的细胞系中，Twist的过表达导致N-钙粘蛋白和纤连蛋白的上调，伴随着E-钙粘蛋白的下调。综上所述，我们的研究结果表明，Twist调节胃癌细胞系的细胞运动和侵袭，可能是通过N-钙粘蛋白和纤连蛋白的产生。(C)2007年爱思唯尔公司All rights reserved.
Twist, a newly found EMT-inducer, has been reported to be up-regulated in those of diffuse-type gastric carcinomas with high N-cadherin level. We show here MKN45, a cell line derived from undifferentiated carcinomas cells, expresses high levels of Twist. Down-regulation of Twist, using an antisense Twist vector in MKN45 cells, inhibits cell migration and invasion, companied with a morphologic changes associated with MET. Suppression of Twist also decreases the expressions of N-cadherin and fibronectin, but not of E-cadherin in MKN45. In contrast, overexpression of Twist in MKN28, a cell line derived from moderate differentiated carcinomas, results in upregulation of N-cadherin and fibronectin, companied with down-regulation of E-cadherin. Taken together, our results suggest that Twist regulates cell motility and invasion in gastric cancer cell lines, probably through the N-cadherin and fibronectin production. (C) 2007 Elsevier Inc. All rights reserved.